Lab Manual
The lab experiments listed below have been professionally authored, with the ability to be modified to your lab curriculum. All labs include step-by-step instructions and required equipment.
These are for informational purposes only. More current and complete directions are included with the downloadable LabScribe software.
Lab Experiments | Equipment Required | Included with following Kits | Goals and Outcomes |
|---|---|---|---|
| Equipment Required | Included with following Kits | Goals and Outcomes | |
TT-01: Tutorial with ECGUse this tutorial to acquaint yourself with the most commonly used features of LabScribe. Additional information about the software is available from the Help menu on the software's Main window. Uses ECG recordings. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully operate both the iWorx A/D converter and the LabScribe software. 2. Students will be able to load the appropriate lab settings group and file, exercise, and attached lab courseware in PDF format for use during lab. 3. Students will be able to attach peripheral devices and transducers to the iWorx A/D converter. 4. Students will be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have become skilled in the workings of the LabScribe software. 2. have been able to successfully use the Load Group function to open the lab exercise and pdf courseware. 3. feel comfortable attaching peripheral transducers to the A/D converter. 4. have used the functions available in the Analysis window to determine values for pulse amplitude and heart rate. |
TT-02: Tutorial with pulseUse this tutorial to acquaint yourself with the most commonly used features of LabScribe. Additional information about the software is available from the Help menu on the software's Main window. Uses pulses sensor. | IX-TA-ROAM, , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully operate both the iWorx A/D converter and the LabScribe software. 2. Students will be able to load the appropriate lab settings group and file, exercise, and attached lab courseware in PDF format for use during lab. 3. Students will be able to attach peripheral devices and transducers to the iWorx A/D converter. 4. Students will be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have become skilled in the workings of the LabScribe software. 2. have been able to successfully use the Load Group function to open the lab exercise and pdf courseware. 3. feel comfortable attaching peripheral transducers to the A/D converter. 4. have used the functions available in the Analysis window to determine values for pulse amplitude and heart rate. |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HH-01: Electrocardiogram (ECG) and Peripheral CirculationIn this experiment, you will record a single lead ECG and the pulse wave in the finger of a subject simultaneously. This exercise will demonstrate the time delay that occurs between the electrical events in the heart and mechanical events in the circulatory system. You will also examine the effects of temperature on peripheral circulation. | IX-TA-ROAM, , ROAM-B2A , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and examine the relationship of the ECG to the peripheral circulation. 2. Students will be able to record and look at the effects of hot and cold on an ECG and pulse in the extremities. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and the amplitudes of various ECG waves. 6. have been able to examine and interpret the effects of hot and cold on peripheral circulation. |
HH-02: ECG and Heart Sounds while listening with a stethoscopeRecording an ECG and listening to heart sounds using a manual stethoscope. | IX-TA-ROAM, , ROAM-B2A , Stethoscope | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and listen to heart sounds using a stethoscope. 2. Students will be able to compare the ECG to the heart sounds and determine when the sounds occur during a cardiac cycle. 3. continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have been able to examine and interpret the heart sounds and when they occur in an ECG recording. |
HH-03: Exercise, the ECG and Peripheral CirculationIn this laboratory you will record the electrocardiogram and the finger pulse from a (healthy) subject. These parameters will be recorded when the subject is at rest and immediately after exercise. | IX-TA-ROAM, , ROAM-B2A , PPG-320 , FT-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and examine the relationship between the ECG and the peripheral circulation. 2. Students will be able to record and look at the effects of exercise on an ECG and pulse in different subjects during the lab period. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and the amplitudes of various ECG waves. 6. have been able to examine and interpret the effects of exercise on ECG and pulse amplitudes and timing. |
HH-04: Six Lead ECGIn this experiment, students will place five electrodes on a subject and be able to record six different views of the subject’s heart. Each view is often referred to as a lead. This is possible because the electrodes are used in different combinations, as either recording or reference electrodes, to create six different views of the heart. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a six-lead Electrocardiogram (ECG) and interpret a six-lead ECG. 2. Students will interpret data looking at the different ECG leads: I, II, III, aVL, aVR, and aVF. 3. Students will be able to calculate amplitudes of the P, R and T waves; the QRS axis; and the heart angle from the data collected during recording. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable six-lead ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, the QRS complex, and answer questions about these waves. 3. be able to calculate the heart angle of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and various ECG amplitudes. |
HH-05B: The Diving Reflex with Respiration rateIn this experiment, students will determine their normal heart rates while resting, holding their breath, and holding their breath as they submerge their faces in cool water. | IX-TA-ROAM, , ROAM-B2A , PPG-320 , RM-204 | Goals1. Students will be able to successfully record a pulse using the plethysmograph. 2. Students will be able to record and look at the effects of apnea, and facial immersion into both room temperature and cold water on the pulse wave. 3. As an optional exercise, students will be able to examine the effects of apnea, and facial immersion into both room temperature and cold water on respiration rate and depth. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable pulse wave and heart rate trace on a resting individual. 2. have recorded a recognizable pulse wave and heart rate trace on an individual during apnea and facial immersion into room temperature and cold temperature water. 3. be able to calculate the pulse rate of an individual from the recorded data and understand the effects of the diving reflex. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. as an optional exercise have been able to examine and interpret the effects of apnea, and facial immersion into both room temperature and cold water on respiration rate and depth. | |
HH-06: Heart Rate VariabilityIn this experiment, a power spectral analysis (PSA) of the heart rate variability (HRV) of a subject will be determined from the subject’s ECG. The analysis involves the mathematical transformation of the ECG record to yield its power spectrum and the analysis of the spectrum to determine the density of defined frequency ranges in the spectrum. The first step in the analysis is to express successive R-R intervals in the record as a function of the time or the heartbeat number in the record. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and examine heart rate variability (HRV) while resting, after exercise and during a psychological test. 2. Students will be able to record and look at the effects of exercise and a stressful test on HRV. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG and be able to calculate the heart rate of an individual from the recorded data. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to interpret data to look at HRV after exercise and during a psychological test. 4. answered questions about HRV and how HRV is influenced during times of “stress”. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
HH-08: AuscultationIn this lab, students will record the heart sounds that occur during the cardiac cycle using a heart sound monitor placed on the four prescribed auscultation areas around the heart. The first heart sound (S1) occurs during the early phase of ventricular contraction and is produced by closing of the atrioventricular valves, which prevents blood flow back into the atria as the ventricle contracts. The second heart sound (S2) occurs when the ventricles relax and is produced by the closing of the semilunar valves, which prevents blood from flowing back into the ventricles from the large blood vessels. The ECG of the subject is recorded as the heart sounds are recorded so that you can visualize when heart sounds occur during the ECG cycle. | IX-TA-ROAM, , ROAM-B2A , HSM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and listen to heart sounds using a heart sounds microphone. 2. Students will be able to compare the ECG to the heart sounds and determine when the sounds occur during a cardiac cycle. 3. Students will understand and be able to locate the 4 major auscultation areas on the chest. 4. Students will monitor ECG and heart sounds under varying conditions. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. have calculated the ratio of the average area to the average duration of the heart sound integrals for the S1 and S2 heart sounds from each auscultation area. 4. have determined the relative amplitude of each heart sound from each auscultation area. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have been able to examine and interpret the heart sounds and when they occur in an ECG recording. |
HH-09: ECG and Heart Sounds using an Electronic StethoscopeIn this lab you will record the ECG and heart sounds from a subject and, with the aid of a stethoscope, hear those characteristic sounds of the heartbeat, typically described as a "lub-dub." These sounds are produced by the closure of the heart valves. | IX-TA-ROAM, , ROAM-B2A , ES-300 | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and listen to heart sounds using an electronic stethoscope. 2. Students will be able to use a stethoscope correctly and accurately. 3. Students will understand and be able to locate the 4 major auscultation areas on the chest. 4. Students will be able to compare the ECG to the heart sounds and determine when the sounds occur during a cardiac cycle. 5. Students will monitor ECG and heart sounds under varying conditions. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. have calculated the ratio of the average area to the average duration of the heart sound integrals for the S1 and S2 heart sounds from each auscultation area. 4. have determined the relative amplitude of each heart sound from each auscultation area. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have been able to examine and interpret the heart sounds and when they occur in an ECG recording. | |
HH-10: 12-Lead Electrocardiograms (ECGs)In this lab, you will record electrocardiograms from Lead I, Lead II, and one of the six chest leads. Lead I will be used as a reference. The augmented limb leads; Lead III, aVR, aVL, and aVF, will be calculated from the reference electrode. The chest lead will be moved to the correct locations on the subjects chest to record each of 6 chest lead positions. The amplitudes and shapes from each chest lead will be compared, and the transition point of each subject will be determined. | IX-TA-ROAM, , IWIRE-ECG12 | Goals1. Students will be able to successfully record and interpret a 12-lead Electrocardiogram (ECG). 2. Students will interpret data looking at the different ECG leads: I, II, III, aVL, aVR, and aVF, and the 6 chest leads. 3. Students will be able to calculate amplitudes of the P, R and T waves; the QRS axis; and the heart angle from the data collected during recording. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable 12-lead ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, the QRS complex, and answer questions about these waves. 3. be able to calculate the heart angle of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and various ECG amplitudes. | |
HH-11: ECG ~ Simulations and ComparisonsIn this experiment, you will record and analyze a three lead ECG directly from a live subject, as well as recording a variety of simulated ECG heart rhythms. These laboratory exercises will demonstrate the differences between normal and abnormal electrical events in the heart. Students can also examine the differences between adult ECG heart rhythms and those of an infant. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record an Electrocardiogram (ECG) both on a live subject and using an ECG Simulator. 2. Students will be able to record a normal sinus rhythm both on the their subject and using the simulator. 3. Students will be able to use the ECG simulator to record abnormal cardiac rhythms. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG with a normal sinus rhythm. 2. be able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. be able to recognize and understand the differences in ECG recordings during abnormal cardiac situations. 5. understand the physiology behind normal and abnormal cardiac rhythms. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and the amplitudes of various ECG waves. |
HH-12: Pulse and Heart Rate Variability (HRV)In this experiment, the subject’s Pulse is recorded for at least a ten-minute period during each exercise. During the recording, an analysis of the ECG is performed by computed functions, and the results are displayed on four additional channels on the Main window: HR, HRV-LP, HRV-HP, HRV-Ratio. | IX-TA-ROAM, , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a pulse plethysmograph and examine heart rate variability (HRV) while resting, after exercise and during a psychological test. 2. Students will be able to record and look at the effects of exercise and a stress-type test on HRV. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded recognizable pulse waves and be able to calculate the heart rate of an individual from the recorded data. 2. be able to interpret data to look at HRV after exercise and during a psychological test. 3. answered questions about HRV and how HRV is influenced during times of “stress”. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HM-01: Grip Strength and the ElectromyogramIn this experiment, students will use a hand dynamometer to measure a subject’s grip strength as the EMG activity of the forearm muscles used to generate the subject’s grip are recorded. The EMG activity will be related to the grip strength by plotting the maximum grip strength as a function the area under the absolute integral of the EMG activity during the muscle contraction. Data recordings will be made from the subject’s dominant and non-dominant forearms, and the relative strength and electrical activity of each forearm will be compared to its circumference. Recordings of prolonged grip strength and forearm EMG activity will also be made to determine the rate of fatigue in the dominant and non-dominant forearms. | IX-TA-ROAM, , ROAM-B2A , FT-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will successfully record electromyograms (EMGs). 2. Students will learn how to calibrate a dynamometer and convert pounds to kilograms. 3. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 4. Students should be able to measure the EMG produced and corresponding muscle force. 5. Students will measure the force produced by the muscle in both the dominant and non-dominant forearms. 6. Students will also study and measure the effect of fatigue on the muscles in the dominant and non-dominant forearms. Comparison of the measurement will also be examined. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between nerve impulses and the resulting EMG recording. 4. have gained understanding of the reasons for different responses in the dominant and non-dominant forearm, and the correlation between fatigue and muscle strength. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-02: Electromyogram Activity in Antagonistic MusclesIn this experiment, students will record EMG activity from muscles on the anterior and posterior sides of the forearm to determine which ones are responsible for flexion and extension of the hand. Recording of EMG activity from these muscles will also be done while a weight is lifted by the hand. In another exercise, students will record EMG activity from the anterior and posterior sides of the lower leg to determine which muscles are active during movements or positioning that are more complex, like leaning forward, standing on toes, or rocking on heels. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from antagonistic muscle groups in both the forearm and lower leg. 2. Students will learn how levers, fulcrums, and load affect the workings of antagonistic muscles. 3. Students will gain an understanding of the muscle groups involved in flexion, extension, dorsiflexion and plantar flexion. 4. Students will use weights to put load on muscles groups while examining changes in the EMG. 5. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 6. Students should be able to measure the EMG produced and corresponding muscle force. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between antagonistic muscles during movement. 4. have gained understanding of the relationship between load and muscle activity. 5. have measured the EMG force difference between muscle groups without and without lifting a weight. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-03: Oculomotor Muscle ActivityIn this experiment, the subject will perform tasks that will generate electrical activity that will alter the standing voltage between the front and back of the eye that is correlated with horizontal eyeball movement. This movement will be obtained by electrodes placed on the skin near the eye. The record of this electrical activity is known as an electroculogram (EOG). | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will successfully record electroculograms (EOGs) from the oculomotor muscle group of the eye. 2. Students will learn how the six oculomotor muscles control eye movement during saccades, pursuit, the vestibular ocular reflex (VOR), and vergence. 3. Students will perform tasks that will generate electrical activity in oculomotor muscles that are unique to each of four different types of eye movement (saccades, VOR, pursuit, and vergence). 4. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EOG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between saccades and reading; pursuit and following a moving target; VOR and head rotation; and vergence and focusing near to far. 4. have measured the EOG amplitude to determine the motion of the subject’s eyes during various oculomotor activities. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-04: Stimulus Response, Work, Summation and Tetanus in Human MusclesIn this experiment, students will use a specialized displacement transducer, the SMT-220 striated muscle transducer, to demonstrate the effect of increasing the stimulus strength on the strength of contraction of a muscle, the effect of increasing weight on twitch amplitude and work of a preloaded muscle, and the effect of increasing the frequency of stimulation on the contraction strength and muscle fatigue. | IX-TA-ROAM, , C-HVS-SL2 , SMT-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, NBK-TR, | Goals1. Students will learn how to apply a stimulus pulse to human muscle to elicit a muscular contraction. 2. Students will record finger twitches to be able to recognize contraction and relaxation times and twitch amplitudes. 3. Students will demonstrate the effect of increasing stimulus strength on the strength of a muscle contraction, the effect of • increasing weight on twitch amplitude and work of a preloaded muscle, • increasing the frequency of stimulation on the contraction strength and muscle fatigue. 4. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 5. Students should be able to measure the EMG produced and corresponding muscle force. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record a simple finger twitch. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between stimulus and muscle twitch amplitudes. 4. understand the concepts of muscle recruitment, fatigue, summation, and tetanus 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-05: Flexibility and Range of MotionIn this experiment, students will use a single-axis goniometer to measure the flexibility and range of motion of the wrist, elbow, ankle, and knee of various subjects. | IX-TA-ROAM, , GN-100 | UHK-TR, UAHK-TR, UBIK-TR, NBK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from antagonistic muscle groups in both the forearm and lower leg. 2. Students will learn how levers, fulcrums, and load affect the workings of antagonistic muscles. 3. Students will gain an understanding of the muscle groups involved in flexion, extension, dorsiflexion and plantar flexion. 4. Students will use a goniometer to measure angle of motion and flexibility of muscle groups. 5. Students will gain an understanding of how the range of motion determines the joint’s functionality. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how flexibility and range of motion are used by physical therapists and athletic trainers when looking at joint dysfunction.. 3. be able to determine the relationship between antagonistic muscles during movement and how that related to range of motion.. 4. understand how flexion and extension of joints with and without weights affects the range of motion of that joint. 5. gain an understanding of why different joints have different flexibilities and ranges of motion in certain directions. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-06: M-wave and Range of Motion with Stimulation of Antagonistic MusclesIn this experiment the Tibialis anterior and Gastrconemius muscles will be used. When stimulated, each muscle generates a compound muscle action potential, known as an M-wave which will be recorded like an electromyogram (EMG). Each M-wave generates a muscle contraction, which can causes flexion. The frequency of stimulation of the muscles will also be increased to demonstrate the effect of mechanical summation on the overall motion of the foot. | IX-TA-ROAM, , ROAM-B2A , GN-100 , C-HVS-SL2 | UHK-TR, UAHK-TR, UBIK-TR, | Goals1. Students will learn how to apply a stimulus pulse to human muscle to elicit a muscular contraction. 2. Students will record finger twitches to be able to recognize contraction and relaxation times and twitch amplitudes. 3. Students will gain an understanding of the muscle groups involved in flexion, extension, dorsiflexion and plantar flexion. 4. Students will use a goniometer to measure angle of motion and flexibility of muscle groups. 5. Students will gain an understanding of how the range of motion determines the joint’s functionality. 6. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record a simple muscle twitch. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between stimulus and muscle twitch amplitudes. 4. understand contraction and relaxation times of muscles. 5. understand how stimulus frequency compares to the ranges of motion for dorsiflexion and plantar flexion of the foot, and flexion and extension of both the wrist, elbow and knee. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-07: Electromyogram (EMG) Activity in Antagonistic Muscles and Range of MotionIn this experiment students will record EMG activity from muscles on the anterior and posterior sides of the forearm to determine which ones are responsible for flexion and extension of the hand. While the wrist joint is being used, range of motion (ROM) will also be recorded. Recording of EMG activity from these muscles and ROM of the joint will also be done while a weight is lifted by the hand. In another exercise, students will record EMG activity from the anterior and posterior sides of the lower leg to determine which muscles are active during movements or positioning that are more complex, like leaning forward, standing on toes, or rocking on heels. ROM of the ankle will also be examined during these parameters - looking at both plantar and dorsi- flexion. | IX-TA-ROAM, , ROAM-B2A , GN-100 | UHK-TR, UAHK-TR, UBIK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from antagonistic muscle groups in both the forearm and lower leg. 2. Students will learn how levers, fulcrums, and load affect the workings of antagonistic muscles. 3. Students will gain an understanding of the muscle groups involved in flexion, extension, dorsiflexion and plantar flexion. 4. Students will use a goniometer to measure angle of motion and flexibility of muscle groups. 5. Students will gain an understanding of how the range of motion determines the joint’s functionality. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how flexibility and range of motion are used by physical therapists and athletic trainers when looking at joint dysfunction.. 3. be able to determine the relationship between antagonistic muscles during movement and how that related to range of motion.. 4. understand how flexion and extension of joints with and without weights affects the range of motion of that joint. 5. gain an understanding of why different joints have different flexibilities and ranges of motion in certain directions. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-08: Electromyogram (EMG) Activity while Arm WrestlingIn this lab, students will use arm wrestling to observe and measure EMG activity while winning and losing at a wrestling match. Electrodes will be placed on the Biceps brachii (upper arm) and Pronator teres (forearm) muscles to observe muscle action as different forces are used to pin their opponent to the table. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from antagonistic muscle groups in the forearm, upper arm and shoulder. 2. Students will learn how levers, fulcrums, and load affect the workings of antagonistic muscles. 3. Students will gain an understanding of the muscle groups involved in flexion and extension while performing a specific task. 4. Students will put load on muscles groups while examining changes in the EMG. 5. Students will gain an understanding of the relationship between the muscle action and function using preselected muscles and then using muscles groups for studying their own hypotheses. 6. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 7. Students should be able to measure the EMG produced and corresponding muscle force. 8. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between antagonistic muscles during movement. 4. have gained understanding of the relationship between load and muscle activity. 5. have measured the EMG force difference between muscle groups while performing a specific task. 6. have designed optional experiments or muscle groups and test varied hypotheses. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 8. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-09: Kinesiology and Electromyogram (EMG) Activity in Targeted MusclesThis lab will allow the grouping of muscles, specifically those in the hip area, and target movements to exercise those individual muscles. Recording EMG activity while doing these exercises will give immediate feedback as to whether the muscle being targeted by a specific exercise is the one that is actually being used. | IX-TA-ROAM, , IWIRE-B3G | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from muscle groups in leg. 2. Students will learn how levers, fulcrums, and load affect the workings of muscles. 3. Students will gain an understanding of the muscle groups involved in flexion, extension, abduction, adduction, etc... 4. Students will use weights to put load on muscles groups while examining changes in the EMG. 5. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 6. Students should be able to measure the EMG produced and corresponding muscle force. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between different muscles during movement. 4. have gained understanding of the relationship between load and muscle activity. 5. have measured the EMG force difference between muscle groups without and without doing a specific activity. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-10: The Electrogastrogram (EGG) and Growling StomachTo record an EGG, electrodes are placed on the skin of the abdomen just below the stomach on the left side of the body. While the subject is lying down and relaxing, a recording is done of the electrical activity of the stomach and/or intestines. The gastric electrical activity is recorded after fasting for approximately 15 minutes, then again after a small meal is eaten for the same length of time. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HM-11: Electromyogram (EMG) for Paired Arm WrestlingIn this lab, students will use arm wrestling to observe and measure EMG activity while winning and losing at a wrestling match. Electrodes will be placed on the Biceps brachii (upper arm) and Pronator teres (forearm) and Deltoid (shoulder) muscles to observe muscle action as different forces are used to pin their opponent to the table. | IX-TA-ROAM, , ROAM-B2A , IWIRE-B3G | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from antagonistic muscle groups in the forearm, upper arm and shoulder. 2. Students will learn how levers, fulcrums, and load affect the workings of antagonistic muscles. 3. Students will gain an understanding of the muscle groups involved in flexion and extension while performing a specific task. 4. Students will put load on muscles groups while examining changes in the EMG. 5. Students will gain an understanding of the relationship between the muscle action and function using preselected muscles and then using muscles groups for studying their own hypotheses. 6. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 7. Students should be able to measure the EMG produced and corresponding muscle force. 8. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between antagonistic muscles during movement. 4. have gained understanding of the relationship between load and muscle activity. 5. have measured the EMG force difference between muscle groups while performing a specific task. 6. have designed optional experiments or muscle groups and test varied hypotheses. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 8. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HM-12: Looks at balance and proprioception.Uses a balance board to look at balance and proprioception. | IX-TA-ROAM, | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | GoalsOutcomes |
HM-13: Uses a box step or aerobic step to look at muscle activity during jumping or other movements.Uses a box step or aerobic step to look at muscle activity during jumping or other movements. | IX-TA-ROAM, , ROAM-B2A | GoalsOutcomes |
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HM-14: Uses the HVS to stimulate abdominal muscles to demonstrate what menstrual cramps feel like.In this lab, students will be using the IXTA simulator to essentially create the “feeling” of menstrual cramps. Students will be measuring pulse and completing tasks while being subjected to cramps to feel what those who have periods endure in their lives. Note that this is not an EXACT replication of cramps – it is similar but cramps come from in the body not outside like the electrical current from the stimulation electrodes. The contracting muscles that the electrical current causes are the replication of the cramps unfortunately the electrical current will be felt due to the nature of the technology. | IX-TA-ROAM, , PPG-320 , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HM-15: Grip Strength, the Electromyogram, and Muscle Oxygen ConcentrationIn this experiment, students will use a hand dynamometer to measure a subject’s grip strength as the EMG activity of the forearm muscles used to generate the subject’s grip are recorded. The EMG activity will be related to the grip strength by plotting the maximum grip strength as a function the area under the absolute integral of the EMG activity during the muscle contraction. Data recordings will be made from the subject’s dominant and non-dominant forearms, and the relative strength and electrical activity of each forearm will be compared to its circumference. Recordings of prolonged grip strength and forearm EMG activity will also be made to determine the rate of fatigue in the dominant and non-dominant forearms. During these experiments, muscle oxygen concentration will also be recorded and analyzed. | IX-TA-ROAM, , ROAM-B2A , FT-220 , MOXY | Goals1. Students will successfully record electromyograms (EMGs). 2. Students will learn how to calibrate a dynamometer and convert pounds to kilograms. 3. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 4. Students should be able to measure the EMG produced and corresponding muscle force. 5. Students will measure the force produced by the muscle in both the dominant and non-dominant forearms. 6. Students will also study and measure the effect of fatigue on the muscles in the dominant and non-dominant forearms. Comparison of the measurement will also be examined. 7. During these experiments, muscle oxygen concentration will also be recorded and analyzed. 8. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between nerve impulses and the resulting EMG recording. 4. have gained understanding of the reasons for different responses in the dominant and non-dominant forearm, and the correlation between fatigue and muscle strength. 5. be able to correlate muscle use with changes in muscle oxygen concentration and cellular respiration. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HN-01: Auditory and Visual ReflexesYou will study the time taken between a stimulus and the response. These reaction time measurements will be made from an individual subjected to harmless visual and sound stimuli. In addition, the effect of priming and prediction will be examined. | IX-TA-ROAM, , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | Goals1. Students will gain an understanding of a reflex arc and how the spinal cord and peripheral nerves function in the human body 2. Students will be able to successfully record responses from subjects to auditory and visual stimuli. 3. Students should be able to measure the response time of their subjects to different cues and relate it to the functioning of the spinal nerves. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to draw a reflex arc. 2. have recorded responses of subjects to both auditory and visual stimuli. 3. determine a subject’s response time to various cues. 4. be able to determine the effect of different types of auditory cues on response time. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HN-02: Stretch Receptors and Reflexes with Reflex HammerStudents will record electromyograms (EMGs), the summation of asynchronous electrical activity (muscle action potentials) in the multiple fibers in the muscle, and use them to determine the time between the stretch of the tendon and the arrival of the motor impulse at the muscle. | IX-TA-ROAM, , ROAM-B2A , PRH-200 | UHK-TR, UAHK-TR, UBIK-TR, | Goals1. Students will successfully trigger and record electromyograms (EMGs) using a reflex hammer and iWorx software, respectively. 2. Students will gain an understanding of the muscles in the leg and how they work and respond to stimuli. 3. Students will gain an understanding of the both the Achilles and patellar stretch reflexes and the reflex arc. 4. Students should be able to measure the conduction times and nerve velocities for the Achilles and patellar reflexes using electromyograms (EMGs). 5. Students will measure the effect of pre-existing tension in the effector muscle, or motor activity in other muscle groups, upon reflex responses. 6. Students will also study the coordination of motor activity in antagonistic muscles. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to draw a reflex arc. 2. have recorded EMG responses of subjects to stimulation of the Achilles and patellar tendons using a reflex hammer. 3. be able to determine the conduction times and nerve velocities using EMG recordings. 4. have measured the effect of pre-existing tension on muscles or muscle groups. 5. have gained understanding of the reasons for different conduction and reaction times of reflexes at different locations on the human body. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HN-03: Human Nerve Conduction VelocityIn this experiment, the ulnar nerve of a subject will be stimulated and the response of a muscle innervated by the nerve will be recorded as an indicator of nerve activity. Three aspects of nerve activity will be studied in this experiment: the relationship between stimulus strength and the amplitude of the nerve/muscle response; the latency of the nerve as a function of the polarity of the stimulus pulse; and the nerve conduction velocity. To measure nerve conduction velocity, the nerve will be stimulated at two different points along its length. Because the distance between the two stimulation points and time difference between muscle responses from those two positions can be measured, the nerve conduction velocity can be calculated. | IX-TA-ROAM, , ROAM-B2A , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will gain an understanding of how a stimulus is applied to a nerve to gain a response. 2. Students will gain an understanding of the Compound Action Potential (CAP) generated by a nerve as a response to a stimulus. 3. Students will be able to successfully measure CAPs of the nerves in the forearm. 4. Students will gain an understanding of summation and the relationship to nerve conduction velocity. 5. Students will understand the relationship between stimulus strength and the amplitude of the nerve/muscle response; the latency of the nerve as a function of the polarity of the stimulus pulse; and the nerve conduction velocity. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have gained an understand of the effect of stimulus strength of the response of the innervated muscle. 2. understand how the amplitude of the CAP relates to threshold. 3. have gained an understanding of how the polarity of the stimulus pulse effects the latency and amplitude of the CAP. 4. have calculated the nerve conduction velocity of the ulnar nerve and understand how that relates to the functionality of the nerve/muscle response. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. |
HN-04: Hand vs. Foot ReactionsIn this lab you will study the time taken between a stimulus and the response. These reaction time measurements will be made from an individual subjected to visual and sound stimuli. Reaction times will be compared when using the hand or the foot as the reactor in these exercises. | IX-TA-ROAM, , EM-220 , FRS-220 | PK-TR, | Goals1. Students will gain an understanding of a reflex arc and how the spinal cord and peripheral nerves function in the human body 2. Students will be able to successfully record responses from subjects to auditory and visual stimuli on reactions of both the hand and the foot. 3. Students should be able to measure the response time of their subjects to different cues and relate it to the functioning of the spinal nerves. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to draw a reflex arc. 2. have recorded responses of subjects to both auditory and visual stimuli. 3. determine a subject’s response time to various cues using the hand vs. the foot - looking at eye-hand vs. eye-foot coordination. 4. be able to determine the effect of using different body parts on response time to various cues. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HN-06: Hoffman Reflex using the Soleus MuscleIn this experiment, the H-reflex test is performed using an isolated stimulator, which will deliver a square-wave pulse of short duration and small amplitude. EMG electrodes record the muscle activity. The reflex is usually a clear wave, called an H-wave (or H-reflex), 28-35 ms after the stimulus. An M-wave, an early response representing direct efferent stimulation, may be seen 3-6 ms after the onset of stimulation. The H-wave is the later response. | IX-TA-ROAM, , ROAM-B2A , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will gain an understanding of how a stimulus is applied to a nerve to gain a response. 2. Students will gain an understanding of the Compound Action Potential (CAP) generated by a nerve as a response to a stimulus. 3. Students will be able to successfully measure CAPs of the nerves in the lower leg, specifically those for the soleus muscle. 4. Students will investigate the H-reflex response and the M-wave response during muscle stimulation. 5. Students will design optional exercises, including but not limited to: ◦ using the Jendrassik maneuver and determine the result on the Hoffman Reflex. ◦ adding a small weight to the foot. ◦ altering the temperature, using either an ice pack or moist heat pack, to determine the H-reflex in both situations. ◦ collecting a maximal M-wave. Calculate the Hmax:Mmax ratio which is the standard for reporting in sports medicine. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have gained an understand of the effect of stimulus strength of the response of the innervated muscle. 2. understand how the amplitude of the CAP relates to threshold. 3. be able to design their own hypothesis and carry out the experiment to record and collect data accurately. 4. understand that different parameters can have varying effect on the CAP action and muscle response. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HN-07: Median Nerve Conduction VelocityThe objective of this laboratory exercise is to measure the nerve conduction velocity of the median nerve. The median nerve is a major nerve of the upper limb. As illustrated, it originates from the medial and lateral cords of the brachial plexus of the spinal cord, specifically C5-T1. The nerve runs parallel to the humerus in the brachial region and through the elbow joint where it enters the forearm between the pronator teres muscle and the biceps tendon. It courses along the longitudinal plane on the anterior surface of the arm before passing through the carpal tunnel to innervate the first 3 ½ digits in the palmar and dorsal regions of the hand. The motor fibers of the median nerve control muscles that regulate movement of these digits. | IX-TA-ROAM, , ROAM-B2A , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will gain an understanding of how a stimulus is applied to a nerve to gain a response. 2. Students will gain an understanding of the Compound Action Potential (CAP) generated by a nerve as a response to a stimulus. 3. Students will be able to successfully measure CAPs of the nerves in the forearm. 4. Students will gain an understanding of summation and the relationship to nerve conduction velocity. 5. Students will understand the relationship between stimulus strength and the amplitude of the nerve/muscle response; the latency of the nerve as a function of the polarity of the stimulus pulse; and the nerve conduction velocity. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have gained an understand of the effect of stimulus strength of the response of the innervated muscle. 2. understand how the amplitude of the CAP relates to threshold. 3. have gained an understanding of how the polarity of the stimulus pulse effects the latency and amplitude of the CAP. 4. have calculated the nerve conduction velocity of the ulnar nerve and understand how that relates to the functionality of the nerve/muscle response. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. |
HN-08: Human To Human InterfaceIn lab, since we do not have the capability to get Iron Man’s suit or 3-D print a prosthetic, we can use one person as the “brain” and one person as the “prosthesis”. This means that one person will have the ability to actually control the movements of the other! | IX-TA-ROAM, , ROAM-B2A , FT-220 , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | GoalsOutcomes |
HN-09: Game Show PhysiologyThis lab will investigate the both the reflex response and the heart rate in subjects who are “under stress” to answer chapter review questions, or any game show questions, accurately. | IX-TA-ROAM, , PPG-320 , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HN-10: Play Games - Hypothesis TestingThe lab activity shown here is based on “Whack-a-Mole” and set to measure reaction time, accuracy and pulse. Add any other channels to measure other physiological concepts. Directions for adding other channels can be found right in the Set Up information. | IX-TA-ROAM, , PPG-320 , RPD-320 , ROAM-B2A , A-RM-220 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HN-11: Visual Reflexes and Color StimulationIn this experiment, students will measure the time between the onset of a color stimulus and the onset of a subject’s response. They will also look at the difference in reaction times in responding to green vs. red. After becoming accustomed to responding to only one color, the subjects will be asked to respond to a specific color (green or red) when sequences that include both colors are presented. The reaction times from these sequences will be compared to determine if latencies change when subjects are asked to “think before they act”. Subjects can also engage in competition – who has the fastest reaction time...and to what color??? | IX-TA-ROAM, , EM-220 , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | GoalsOutcomes |
HN-12: Nerve Velocity and Hand DominanceThe effect of handedness on nerve conduction has not been fully studied and this lab aims to draw some conclusion to this question: Does the dominant limb have both faster reaction time and faster nerve conduction velocity than the non-dominant limb? | IX-TA-ROAM, , ROAM-B2A , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | GoalsOutcomes |
HN-13: Conditioned Response to StimuliStudents will study the time taken between a stimulus and the response. These reaction time measurements will be made from an individual shown a neutral visual stimulus and then to that same neutral stimulus when a small shock is applied if the reaction is not fast enough. | IX-TA-ROAM, , EM-220 , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, NBK-TR, | GoalsOutcomes |
HN-14A: Senses - Eye and EarUsing models connected to a computer interface can actually allow us to see the action potentials generated by the eye and the ear based on what colors there are "seeing" and sounds they are "hearing". Spike sorting is used to analyze the data. | IX-TA-ROAM, , NMD-SYS | GoalsOutcomes |
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HN-14B: Senses - Eye and EarUsing models connected to a computer interface can actually allow us to see the action potentials generated by the eye and the ear based on what colors there are "seeing" and sounds they are "hearing". Data analysis uses the spike sorting module. | IX-TA-ROAM, , NMD-SYS | GoalsOutcomes |
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| Equipment Required | Included with following Kits | Goals and Outcomes | |
HC-01: Blood Pressure, Peripheral Circulation and Body PositionMeasuring blood pressure using a blood pressure cuff and a stethoscope takes a great deal of practice. To assist students in learning the technique of taking blood pressures with a stethoscope, a pulse plethysmograph will be used to record the appearance and disappearance of pulsatile blood flow in the artery that indicate when the pressures in the cuff are equal to the systolic and diastolic blood pressures. In addition to learning to measure the blood pressure and comparing blood pressures from different subjects, the effects of cuff location, body position, and arm position will be examined. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph, and blood pressure using a non-invasive blood pressure cuff (sphygmomanometer). 2. Students will be able to interpret data from these recordings and understand the difference between systolic and diastolic blood pressure. 3. Students will look at the effects of different cuff and body positions on pulse and blood pressure. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated a non-invasive blood pressure cuff. 2. have recorded recognizable pulse and blood pressure waves and be able to calculate the pulse rate and blood pressure of an individual from the recorded data. 3. have been able to interpret the effects of different cuff and body positions on both pulse and blood pressure. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
HC-02: Blood Pressure, Peripheral Circulation and Imposed ConditionsThis experiment is composed of a long-term exercise and a series of short-term exercises. The long-term exercise examines the effects of food additives on heart rate, blood pressure and peripheral circulation. The short-term exercises examine the effects of apnea, exercise, and temperature on blood pressure and peripheral circulation. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph, and blood pressure using a non-invasive blood pressure cuff (sphygmomanometer). 2. Students will be able to interpret data from these recordings and understand the difference between systolic and diastolic blood pressure. 3. Students will look at the effects of imposed conditions doing either short- or long- term experiments. The effects of food additives, exercise, apnea and temperature changes may be examined. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated a non-invasive blood pressure cuff. 2. have recorded recognizable pulse and blood pressure waves and be able to calculate the pulse rate and blood pressure of an individual from the recorded data. 3. have been able to interpret the effects of different imposed conditions on both pulse and blood pressure. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
HC-03: Pulse Wave VelocityIn this experiment, we will determine the pulse wave velocity in the artery. In general, pulse wave velocity increases when the cross-sectional area of the artery decreases or the distensibility or compliance of the artery decreases. Some typical pulse wave velocities in normal subjects are: 3 to 5 meters/second in the aorta; 7 to 9 meters/second in the subclavian or femoral artery; 15 to 40 meters/second in small arteries. The artery studied in this experiment shows pulse wave velocities between 8 and 13 meters/second. | IX-TA-ROAM, , PPG-320 , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph and a three-lead electrocardiogram (ECG). 2. Students will be able to interpret data from these recordings and understand the amplitudes and values of the ECG waves. 3. Students calculate pulse wave velocity from the ECG and pulse recording data in a resting subject and in subjects after exercise. 4. Students can perform an optional exercise to determine the effect of different temperatures on the pulse wave velocity. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully recorded pulse waves and an ECG. 2. have been able to calculate the pulse rate and ECG amplitudes of an individual from the recorded data. 3. have been able to calculate the normal resting pulse wave velocity (PWV) and the PWV after hand exercises. 4. been able to calculate the normal resting pulse wave velocity (PWV) and the PWV after the forearm has been exposed to different temperatures (Optional). 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
HC-04: Pulse Contour AnalysisWhen a plethysmograph is placed on a subject’s finger, the pulse wave generated at the site of the plethysmograph has two components. The first component in the pulse wave is caused by systolic pressure wave that is directly transmitted from the root of the aorta down the artery to the finger. The second component in the pulse wave is the diastolic component, which is the pressure wave from the lower body reflected back up the aorta. The reflected wave then travels down the arm and to the finger with the plethysmograph. The time difference between the peaks of the two components, known as the reflection time, is inversely proportional to the arterial stiffness. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph and blood pressure using a non-invasive blood pressure cuff (sphygmomanometer). 2. Students will be able to interpret data from these recordings and understand the difference between systolic and diastolic blood pressure. 3. Students will determine the arterial stiffness, vascular tone, and blood pressures of individual subjects. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated a non-invasive blood pressure cuff. 2. have recorded recognizable pulse and blood pressure waves and be able to calculate the pulse rate and blood pressure of an individual from the recorded data. 3. interpret the collected data to determine the Student Stiffness Index (SSI) of the subject’s major arteries. 4. determine the Student Reflection Index (SRI), the indicator of vascular tone in the subject’s large vessels. 5. understand systolic and diastolic blood pressure and make a determination as to whether the subject is hypo-, hyper- or normo-tensive. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
HC-05: Body Position, Exercise and Cardiac OutputIn this experiment, students will use a simple, non-invasive, pulse-pressure technique, in conjunction with an age-based equation, to determine the cardiac outputs of a subject in different postures and in different stages of recovery from exercise. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph and blood pressure using a non-invasive blood pressure cuff (sphygmomanometer). 2. Students will be able to interpret data from these recordings and understand the difference between systolic and diastolic blood pressure. 3. Students will determine the cardiac output and stroke volume of a subject in various body positions. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated a non-invasive blood pressure cuff. 2. have recorded recognizable pulse and blood pressure waves and be able to calculate the cardiac output and stroke volume of an individual from the recorded data. 3. interpret the collected data to determine the systolic and diastolic pressures, and cardiac output of the subject in the reclining, sitting, and standing positions. 4. compare systolic and diastolic pressures, and cardiac output of the subject in the reclining, sitting, and standing positions. 5. understand systolic and diastolic blood pressure and make a determination as to whether the subject is hypo-, hyper- or normo-tensive. 6. compare blood pressures and cardiac output of a subject at various times after exercise. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HE-01: Metabolism and Thermal Response to ExerciseIn this experiment, human subjects will be monitored for their metabolic and thermal responses to a progressively dynamic exercise routine. In this type of routine, periods of exercise with increasing intensity are followed by periods of recovery. Changes in the heart rate, core temperature, skin temperature, and active sweat gland density of the subjects will be recorded and used to determine how the expenditure of energy, mechanical efficiency, heat storage, and evaporative heat loss by each subject changes with increased exercise. Since some subjects will be doing the experiment while wearing heavier clothing than others, a reduction in heat loss through radiation, convection, conduction, and evaporative cooling should cause the heat storage of these subjects to be greater than the lightly dressed subjects. | IX-TA-ROAM, , PPG-320 , TM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a pulse using the plethysmograph. 2. Students will be able to calibrate the temperature sensor to accurately measure body skin temperature. 3. Students will be able to examine sweat gland density of subjects at rest. 4. Students should be able to measure the changes in the heart rate, skin temperature, core temperature, and active sweat gland density of subjects during exercise and recovery from exercise. 5. Students will be able to perform a variety of mathematical calculations to determine the amount of work performed, energy used, oxygen consumed, net mechanical efficiency, heat storage, and evaporative heat loss during the course of the experiment. 6. Students will then determine a subject’s metabolic and thermal response at rest and during exercise; and calculate relative cardiac health by looking at recovery from exercise. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded both a recognizable pulse wave and skin temperature trace on a resting individual. 2. have recorded a recognizable pulse wave and skin temperature trace on an individual during and after exercise. 3. be able to determine the pulse rate of an individual from the recorded data and understand the effects of exercise on pulse rate and body temperature. 4. calculate net mechanical efficiency and evaporative heat loss in order to understand the metabolic and thermal responses to exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. 7. as an additional analysis, have calculated the subject’s relative cardiac health by examining the time it takes the subject’s heart rate to return to normal after exercising. |
HE-02: Recovery from ExerciseIn this experiment, the heart rates of human subjects will be measured as they recover from a three minute period of stepping. The heart rate at a minute into recovery, and the time it takes the subject’s heart rate to return to the resting level, will be used as measures of the subject’s level of physical fitness. | IX-TA-ROAM, , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a pulse using the plethysmograph. 2. Students should be able to measure the changes in the heart rate during exercise and recovery from exercise. 3. Students will be able to determine a subject’s overall “Fitness Rating” based on the time it takes the heart rate to return to normal after exercise. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable pulse wave at rest. 2. have recorded a recognizable pulse wave on an individual during and after exercise. 3. be able to determine the pulse rate of an individual from the recorded data and understand the effects of exercise on pulse rate. 4. determine a subject’s overall fitness and heart health after examining pulse rate recovery from exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HE-12: Targeted Exercise with Wireless Electrocardiogram (ECG)This lab allows students to calculate their target heart rate, and perform a variety of different exercise routines to see what type of exercises maintains target heart rate the best. Have students choose: running, cycling, playing a game of basketball, doing yoga, weight lifting, anything that would allow them to exercise and move. Because the unit is wireless, you can send the students out and about and report back in an hour for data analysis of the different forms of exercise chosen. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HE-04: RMR and Submax VO2(RER) TestingUsing the Metabolic Cart (gas analzyer) students will look at RMR (Resting metabolic rate or basal metabolic rate if the subject has fasted for 12 hours. Generally a resting test.) or Submax VO2 RER through resting or exercise testing. | IX-TA-ROAM, , iWire-GA1 , A-GAK-201-ES , A-CS-3L , A-FH-1000 | HEK-TR, | Goals1. Students will learn how to use and calibrate an O2/CO2 Gas Analyzer. 2. Students will learn how to measure breathing parameters using a spirometer and mixing chamber. 3. Students will use spirometry data to measure VO2, VCO2, and RER. 4. Students will calculate the proportion of fat and carbohydrates utilized while the subject was resting, hyperventilating, recovering from hyperventilation, and recovering from light or moderate exercise. 5. Students will measure oxygen consumption and use four formulas to determine the subject’s heat production, and predicted and observed metabolic rates at the time of the experiment. 6. Students will also determine the metabolic rate of the subject after recovering from moderate exercise. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable breathing pattern using a spirometer. 2. have recorded recognizable expired oxygen and expired carbon dioxide curves. 3. be able to determine relative VO2 and VCO2. 4. be able to determine the RER and RMR from their subject at rest and during various testing protocols. 5. be able to understand how VO2, VCO2, RER and RMR are affected by changes in breathing patterns. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HE-10: Aerobic Fitness Testing - full VO2 max testing using a variety of protocols.Using the Metabolic Cart (gas analzyer) students will look at RMR (Resting metabolic rate or basal metabolic rate if the subject has fasted for 12 hours. Generally a resting test.) or Submax VO2 RER through resting or exercise testing. | IX-TA-ROAM, , iWire-GA1 , A-GAK-201-ES , A-CS-3L , A-FH-1000 | HEK-TR, | Goals1. Students will learn how to use and calibrate an O2/CO2 Gas Analyzer. 2. Students will learn how to measure breathing parameters using a spirometer. 3. Students will use spirometry data and breath by breath analysis to measure VO2 and VCO2 during various exercise activities up to VO2max and/or completion of a “stress” test. 4. Students will measure the heart rate of a subject while he or she is resting while exercising at various levels of intensity from easy to moderate to full VO2max protocols. 5. Students will determine the subject’s RER, proportion of energy sources utilized, and effectiveness of cardiac fitness. 6. Students will learn to choose the correct Fitness Protocols based on the fitness level of the subject being tested. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable breathing pattern using a spirometer. 2. have recorded recognizable expired oxygen and expired carbon dioxide curves. 3. be able to determine relative VO2 and VCO2 and VO2max. 4. be able to determine anaerobic threshold. 5. be able to determine the RER and cardiac fitness from their subject at rest and during various levels of testing protocols. 6. be able to understand how VO2, VCO2 and RER are affected by changes in exercise levels. 7. be able to understand the physiology involved in using different energy sources during exercise. 8. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 9. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HE-13: Fat burning zoneThis lab uses the Metabolic Cart (gas analyzer) to figure out if there really is a Fat-Burning zone. | IX-TA-ROAM, , iWire-GA1 , A-GAK-201-ES , IX-ANT-SCOSCHE , A-FH-1000 | HEK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HS-01: Breathing Parameters at Rest and After ExerciseIn this lab you will measure lung volume parameters in a subject at rest and immediately after exercise, when the body’s demands for oxygen have been elevated. | IX-TA-ROAM, , A-FH-300 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, UBIK-TR, | Goals1. Students will be able to successfully record respiratory cycles. 2. Students should be able to measure respiration volumes including: tidal volume, reserve capacities, vital capacity, and be able to calculate overall lung volume. 3. Students will be able to determine the difference in lung volumes of a subject at rest, immediately after exercise, and up to a few minutes after exercise. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable respiratory cycle at rest. 2. have recorded recognizable respiratory cycles on an individual immediately after exercise and a few minutes after exercise. 3. be able to determine the respiratory volumes of an individual from the recorded data and understand the effects of exercise on lung volumes. 4. determine a subject’s overall fitness and lung health after examining breathing rate recovery from exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HS-02: Breathing and GravityIn this experiment, students will examine the effects of gravity on breathing by measuring the differences in lung volumes of a resting subject while he or she is sitting, standing, or lying down. | IX-TA-ROAM, , A-FH-300 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, UBIK-TR, | Goals1. Students will be able to successfully record respiratory cycles. 2. Students should be able to measure respiration volumes including: tidal volume, reserve capacities, vital capacity, and be able to calculate overall lung volume. 3. Students will examine the effects of gravity on breathing by measuring the differences in lung volumes of a resting subject while he or she is sitting, standing, or lying down. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable respiratory cycle at rest. 2. have recorded recognizable respiratory cycles on an individual while he or she is sitting, standing and lying down. 3. be able to determine the respiratory volumes of an individual from the recorded data and understand the effects of gravity on lung volumes. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HS-03: Factors that Affect Breathing PatternsIn this experiment, students will examine how other factors, like concentrating on the completion of a task or sitting up quickly, influence breathing and lung volumes. | IX-TA-ROAM, , A-FH-300 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, UBIK-TR, | Goals1. Students will be able to successfully record respiratory cycles. 2. Students should be able to measure respiration volumes including: tidal volume, reserve capacities, vital capacity, and be able to calculate overall lung volume. 3. Students will examine how factors, like concentrating on the completion of a task or sitting up quickly, influence breathing. 4. Students will record and measure lung volumes during these tasks and answer questions based on the data collected. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable respiratory cycle at rest. 2. have recorded recognizable respiratory cycles on an individual while performing different tasks like concentrating on the completion of a problem or sitting up quickly. 3. be able to determine the respiratory volumes of an individual from the recorded data and understand the effects of these tasks on lung volumes. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HS-04: Lung Volumes and Heart RateIn this experiment, you will determine the heart rate and RSA prominence of a subject breathing at rest. You will also determine the effect of apnea, different inhalation volumes, and the movement of the muscles involved in breathing on heart rate. To make valid comparisons from exercise to exercise, the exercises in this lab need to be performed by the same subject. | IX-TA-ROAM, , A-FH-300 , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, UBIK-TR, | Goals1. Students will be able to successfully record respiratory cycles and pulse waves. 2. Students should be able to measure tidal volume and be able to calculate both breathing and heart rate. 3. Students will determine the heart rate and respiratory sinus arrhythmia (RSA) prominence of a subject breathing at rest. 4. Students will also determine the effect of apnea, different inhalation volumes, and the movement of the muscles involved in breathing on heart rate. 5. Students will record and measure lung volumes during these tasks and answer questions based on the data collected. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded recognizable respiratory cycles and pulse waves. 2. be able to measure tidal volume amplitudes, and calculate breathing and heart rate. 3. be able to determine the effects of apnea, use of different muscle groups and inhalation volumes on heart rate. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HS-05: Breathing Techniques and Heart RateIn this experiment, you will determine the heart and breath rates of a subject breathing normally and with different breathing techniques, which include shallow abdominal breathing, rapid bellows breathing, and deep abdominal breathing. | IX-TA-ROAM, , PPG-320 , A-RM-220 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record respiratory cycles and pulse waves. 2. Students should be able to measure tidal volume and be able to calculate both breathing and heart rate. 3. Students will determine the heart rate and respiratory sinus arrhythmia (RSA) prominence of a subject breathing at rest. 4. Students will also determine the effect of apnea, shallow abdominal breathing, rapid bellows breathing, and deep abdominal breathing on heart rate. 5. Students will record and measure lung volumes during these tasks and answer questions based on the data collected. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded recognizable respiratory cycles and pulse waves. 2. be able to measure and calculate breathing and heart rates. 3. be able to determine the effects of apnea, use of different muscle groups and inhalation volumes on heart rate. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HS-08: Restrictive and Obstructive Airway DiseasesIn this lab, students will use a spirometer to measure lung volumes while simulating both restrictive and obstructive airway diseases. It is important that the students who participate in the breathing exercises are healthy and do not have any of the characteristic diseases. | IX-TA-ROAM, , A-FH-300 , A-SP-RS | Goals1. Students will be able to successfully record resting respiratory cycles. 2. Students should be able to measure tidal volume and be able to calculate breathing rate. 3. Students should be ale to use the Analysis window to calculate Max and Min dv/dt. 4. Students will determine FVC, FEV1 and FEV3 and the FEV/FVC ratios. 5. Students will also determine the effect of breathing with a corset on and breathing through a narrowed airway. 6. Students will record and measure lung volumes during these tasks and answer questions based on the data collected. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded recognizable respiratory cycles. 2. be able to measure and calculate breathing rates, breathing parameters and specific ratios. 3. be able to determine the effects of restriction and obstruction on lung volumes and breathing rate. 4. understand the differences between restrictive and obstructive airway diseases. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HP-01: The Electroencephalogram (EEG)Subjects will perform tasks that will allow observers to discriminate between the two EEG wave types, Alpha and Beta, which are present in EEG records of subjects that are awake. | IX-TA-ROAM, , ROAM-B2A , A-HB-ROAM | UHK-TR, UAHK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to collect electroencephalogram (EEG) signals from the left and right cerebral hemispheres. 2. Students will learn to recognize common EEG artifacts caused by movements such as eye blinks, facial muscle contractions, and head movement. 3. Students should be able to recognize and analyze Alpha and Beta EEG patterns associated with closed and open eye conditions; 4. Students will observe the Alpha block. 5. Students will test an experimental hypothesis about relative levels of Alpha and Beta EEG waves in each hemisphere in two psychological states. 6. Students will test an experimental hypothesis about personality and EEG. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded recognizable EEG traces for Alpha and Beta waves on both the right and left cerebral hemispheres. 2. be able to recognize common EEG artifacts. 3. be able to determine the effects of eye conditions (open or closed) and Alpha block on an EEG recording. 4. have tested a hypothesis and reached a conclusion about psychological states and brain hemisphere dominance. 5. have taken personality profile test and explored a hypothesis about EEG and personality. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-02: Galvanic Skin Response and EmotionMeasurements of skin conductance will be used to analyze psychophysiological responses. The tonic level of skin conductance, the frequency of spontaneous conductance responses, and the habituation of the skin conductance response will be recorded and analyzed. | IX-TA-ROAM, , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure the tonic level of skin conductance, the frequency of spontaneous conductance responses, and the habituation of the skin conductance response. 2. Students will observe and measure the galvanic skin response (GSR) as an orienting response to being asked neutral content questions. 3. Students will observe and measure the GSR in response to questions with emotional content. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded GSR traces. 2. be able to recognize changes in the GSR as a response to neutral content or emotional content questions. 3. be able to determine and understand the effects of these questions on an individual’s GSR. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-03: Galvanic Skin Response and Deception, Coginitive Compliance and VigilanceThis experiment contains four exercises that are a continuation of GSR-A and use changes in skin conductance to measure subjects’ psychophysiological responses to additional tasks. | IX-TA-ROAM, , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure the tonic level of skin conductance, the frequency of spontaneous conductance responses, and the habituation of the skin conductance response. 2. Students will observe and measure the galvanic skin response (GSR) as an orienting response to being asked neutral content questions. 3. Students will test an experimental hypothesis about deliberate deception, guilty knowledge, and the amplitude of the GSR. 4. Students will test an experimental hypothesis about cognitive complexity and the latency of the GSR. 5. Students will test an experimental hypothesis about personality, vigilance, and the lability of skin conductance levels. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded GSR traces. 2. be able to recognize changes in the GSR as a response to neutral content questions and habituation. 3. come to a conclusion after testing the hypothesis on deliberate deception and guilty knowledge. This will allow students to gain an understanding of the nature of Polygraph Tests. 4. come to a conclusion with regard to the hypotheses about cognitive complexity, personality and vigilance; and how these play a role in the GSR of individuals. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-04: Skin Temperature, Stress, Calming and EmbarrassmentStudents will perform a set of exercises that will measure baseline skin temperature and record the data,measure changes in skin temperature during a mild psychosocial stressor (mental arithmetic), measure the effect of calming mental imagery in a biofeedback paradigm on skin temperature and test an experimental hypothesis about embarrassability, blushing, and gender. | IX-TA-ROAM, , PPG-320 , C-GSR-320 , TM-220 | UHK-TR, UAHK-TR, UBIK-TR, | Goals1. Students will learn to measure and record skin temperature. 2. Students will observe and measure the changes in skin temperature during a mild psychosocial stressor, a mental arithmetic test. 3. Students will measure the effect of calming mental imagery in a biofeedback paradigm on skin temperature. 4. Students will test an experimental hypothesis about embarrassability and, blushing and gender. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated the temperature probe and recorded skin temperature traces. 2. be able to recognize changes in skin temperature as a result of a mild stressor and when using calming mental imagery. 3. come to a conclusion with regard to the about gender and embarrassability, using changes in skin temperature as the correlating factor. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-04: Skin Temperature, Stress, Calming and EmbarrassmentStudents will perform a set of exercises that will measure baseline skin temperature and record the data,measure changes in skin temperature during a mild psychosocial stressor (mental arithmetic), measure the effect of calming mental imagery in a biofeedback paradigm on skin temperature and test an experimental hypothesis about embarrassability, blushing, and gender. | IX-TA-ROAM, , PPG-320 , IWIRE-B3G , TM-220 | AHK-TRiw, UAHK-TR, | Goals1. Students will learn to measure and record skin temperature. 2. Students will observe and measure the changes in skin temperature during a mild psychosocial stressor, a mental arithmetic test. 3. Students will measure the effect of calming mental imagery in a biofeedback paradigm on skin temperature. 4. Students will test an experimental hypothesis about embarrassability and, blushing and gender. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated the temperature probe and recorded skin temperature traces. 2. be able to recognize changes in skin temperature as a result of a mild stressor and when using calming mental imagery. 3. come to a conclusion with regard to the about gender and embarrassability, using changes in skin temperature as the correlating factor. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-05A: Heart Rate and Blood PressureStudents will collect and analyze heart rate and blood pressure data during a baseline period, a mild cognitive stressor (spelling words backwards aloud) and a vigilance reaction time task. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure and record heart rate and blood pressure as a baseline measurement. 2. Students will collect data and analyze heart rate and blood pressure changes during a stressful task and during a reaction time test. 3. Students will test a hypothesis that persons with high perceived shyness and behavioral inhibition have lower Vagal tone than persons with low perceived shyness. 4. Students will learn how these measurements coordinate with heart rate and breathing. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated the blood pressure cuff (sphygmomanometer) and recorded blood pressure and pulse. 2. be able to recognize changes from baseline measurements in blood pressure and pulse rate during a stressful task and a reaction time test. 3. come to a conclusion with regard to these changes. 4. come to a conclusion about shyness and the relationship with heart rate and breathing, and how this corresponds to vagal tone. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-05B: Personality and Vagal ToneStudents will collect and analyze heart rate and blood pressure data during a baseline period, a mild cognitive stressor (spelling words backwards aloud) and a vigilance reaction time task.. | IX-TA-ROAM, , PPG-320 , A-RM-220 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure and record heart rate and respiration rate as a baseline measurement. 2. Students will collect data and analyze heart rate and respiration rate changes during a stressful task and during a reaction time test. 3. Students will test a hypothesis that persons with high perceived shyness and behavioral inhibition have lower Vagal tone than persons with low perceived shyness. 4. Students will learn how these measurements coordinate with heart rate and breathing. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully recorded pulse and respiration. 2. be able to recognize changes from baseline measurements in pulse and respiration rates during a stressful task and a reaction time test. 3. come to a conclusion with regard to these changes. 4. come to a conclusion about shyness and the relationship with heart rate and breathing, and how this corresponds to vagal tone. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-05C: Vigilance and Reaction TimeStudents will collect and analyze heart rate and blood pressure data during a baseline period, a mild cognitive stressor (spelling words backwards aloud), a vigilance reaction time task. | IX-TA-ROAM, , PPG-320 , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure and record heart rate as a baseline measurement. 2. Students will collect data and analyze heart rate changes during a stressful task and during a reaction time test. 3. Students will test a hypothesis that persons with high perceived shyness and behavioral inhibition have lower Vagal tone than persons with low perceived shyness. 4. Students will learn how these measurements coordinate with heart rate. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully recorded pulse. 2. be able to recognize changes from baseline measurements in pulse rate during a stressful task and a reaction time test. 3. come to a conclusion with regard to these changes. 4. come to a conclusion about shyness and the relationship with heart rate, and how this corresponds to vagal tone. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-05D: Cynicism-Hostility and the Hot ReactorStudents will collect and analyze heart rate and blood pressure data during a baseline period, a mild cognitive stressor (spelling words backwards aloud) and a vigilance reaction time task. | IX-TA-ROAM, , PPG-320 , BP-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will participate anonymously in a personality assessment for the cynicism/hostility personality trait. 2. Students will learn to measure and record heart rate and blood pressure as a baseline measurement. 3. Students will collect and analyze heart rate and blood pressure during a social issues debate and during a recovery to baseline period. 4. Students will test hypotheses about personality and changes in heart rate and/or blood pressure that may have occurred during the debate. 5. Students will learn how these measurements coordinate with being a “hot reactor”. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have taken a personality profile test specifically designed to gauge hostile reactions to certain questions. 2. have successfully calibrated the blood pressure cuff (sphygmomanometer) and recorded blood pressure and pulse. 3. learn about current social issues, proper debate procedures and presenting in front of their peers. 4. be able to recognize changes from baseline measurements in blood pressure and pulse rate during a debate on social issues. 5. come to a conclusion about personality with regard to these changes. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-06: Cell Phone AddictionMeasure physiological changes, such as heart rate and blood pressure, when having/ not having the phone | IX-TA-ROAM, , PPG-320 , BP-220 | UHK-TR, UAHK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure and record pulse, HR and blood pressure. 2. Students will observe and measure the changes in a variety of during a mild stressor - removing cell phones. 3. Students can use this lab to test an experimental hypothesis of their own design. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully recorded pulse, HR and blood pressure. 2. be able to recognize changes in parameters as a result of a mild stressor and looking at length of time with stressor applied. 3. have tested and analyzed their own hypothesis and drawn conclusions using statistical analyses. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-08: The Galvanic Skin Response (GSR) and Investigation into CheatingStudents will use measurements of skin conductance to analyze psychophysiological responses. The tonic level of skin conductance, the frequency of spontaneous conductance responses, and the habituation of the skin conductance response will be recorded to determine if a subject has been ‘cheating’ on quizzes. | IX-TA-ROAM, , PPG-320 , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure the tonic level of skin conductance, the frequency of spontaneous conductance responses, and the habituation of the skin conductance response. 2. Students will observe and measure the galvanic skin response (GSR) as an orienting response to being asked neutral content questions. 3. Students will observe and measure the GSR in response to questions with emotional content and about performing a certain task (lying or telling the truth). 4. Students will measure skin conductance and form conclusions about “lie detector” tests based on the lab experiment. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded GSR traces. 2. be able to recognize changes in the GSR as a response to neutral content or emotional content questions. 3. be able to determine and understand the effects of these questions on an individual’s GSR. 4. understand the nature of “lie detector” tests and be able to articulate whether these tests are accurate or inaccurate based on data collected. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-09: Facial Expression Electromyograms (EMG) and EmotionStudents will record and analyze facial EMGs using an iWorx recorder while looking at a variety of images known to produce emotional responses. Students will be viewing still images, but this exercise can be extended to include both video and responses when listening to music. This lab experiment is also fully customizable to allow students to observe a wide range of parameters to support the idea that facial EMGs are a valid method for determining emotional response. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will successfully record electromyograms (EMGs) from facial muscle groups, especially those involved in smiling and frowning. 2. Students will gain an understanding of the muscle groups involved in making certain facial expressions. 3. Students will study the subject’s emotional response while looking at a series of images based on EMG activity. 4. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 5. Students should be able to measure the EMG produced and corresponding muscle activity. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between EMG activity and smiling or frowning. 4. have gained understanding of the relationship between emotion and certain muscle activity. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-10: Visual Evoked Potentials (VEP)Students will watch a series of flashing lights and images while testing each eye individually and results will be measured by the length of time it took the brain to respond to the stimuli. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will set up equipment and image generator to be able to record electrical activity generating visual evoked potentials (VEPs). 2. Students will learn how to record VEP activity from the subject while the eyes are closed. 3. Students will identify the VEP pattern for both the left and right eyes while looking at a flashing checkerboard. 4. Students will identify the VEP pattern for the both the left and right eyes while looking at a rotating dartboard pattern. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record a VEP from both left and right eyes. 2. understand how nerves send electrical signals to cause a visual response. 3. be able to determine the relationship between VEP activity and right vs. left eyes. 4. be able to determine the difference in VEP activity using a flashing checkerboard and a rotating dartboard. 5. have gained understanding of the latency and response time. 6. understand what is happening physiologically during the latent period and evoked potential. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 8. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-12: Rubber Hand IllusionStudents will perform the rubber hand illusion on different subjects to determine any changes in various physiological parameters. A background questionnaire is initially given to participants, then the rubber hand illusion is conducted, and physiological measures of body schema changes are recorded. The subjects’ subjective experiences are further explained in a survey that immediately follows the illusion. | IX-TA-ROAM, , ROAM-B2A , C-GSR-320 , PPG-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-13: The Gaze Cue ParadigmThis experiment is a gaze-cueing paradigm. The typical finding is that you are faster in the congruent condition as compared to the incongruent condition, even though the direction of gaze is not predictive of the target location. This shows that our attention is automatically guided by other people’s gaze, even in situations where this doesn’t serve any purpose | IX-TA-ROAM, , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | GoalsOutcomes |
HP-13A: The Gaze Cue Paradigm using Eye Tracker systemThis experiment uses the Eye-Tracking System, which allows analysis of other data by actually being able to “see” when the subject’s eyes focused on the object in question – the target letter ‘H’. It will also allow determination of how long it took the subject to recognize the target after their eyes actually focused on it, and if the subject pressed the event marker when they focused on the correct target. | IX-TA-ROAM, , EM-220 , A-iTRAX | GoalsOutcomes |
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HP-14: Hypothesis-driven Biofeedback Lab/Research StudyThis lab is meant to be a “hypothesis-driven” lab where students will come up with a technique or techniques they would like their subject to try in order to alter some aspect of the subject’s physiological parameters. This can include: reducing heart rate, sweating, altering body temperature and/or breathing rate. Parameters can be added or removed based on what the goal of the hypothesis is. | IX-TA-ROAM, , ROAM-B2A , C-GSR-320 , PPG-320 , BP-220 , A-RM-220 , A-HB-ROAM | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | Goals1. Students will learn to measure and record skin temperature, GSR, pulse, and other parameters. 2. Students will observe and measure the changes in a variety of during a mild stressor - heat, cold, imagery, etc.... 3. Students will measure the effect of calming mental imagery in a biofeedback paradigm on various physiological parameters. 4. Students will test an experimental hypothesis of their own design. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated the temperature probe and recorded skin temperature, GSR, pulse and other traces. 2. be able to recognize changes in parameters as a result of a mild stressor and when using calming mental imagery. 3. have tested and analyzed their own hypothesis and drwan conclusions using statistical analyses. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. |
HP-20: Relaxation and MusicListening to relaxing music to control tension. What is the "most relaxing song?" This can be tested right in the lab by recording the subject’s baseline values and then recording while they are listening to the song “Weightless”. You can also compare these “Top 10” relaxing songs to see if one really does work better than the others. | IX-TA-ROAM, , C-GSR-320 , PPG-320 , A-RM-220 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-21: Vacation and RelaxationIn this lab you can test whether a “vacation” can reduce the impact of stress. By looking at a series of stress-inducing images or performing a stressful task, you can simulate that feeling in your subjects. Then have them look at and visualize relaxing, vacation-type images: a day at the beach, a lovely sunset, etc…. | IX-TA-ROAM, , ROAM-B2A , PPG-320 , A-RM-220 , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-22: Anticipation & AnxietyStudents will measure pulse and heart rate, skin temperature and respiration rate while waiting for something to happen (perhaps waiting for a scary video clip, or virtually riding a rollercoaster and waiting for that first drop) and then after the anticipation is over. Different anticipatory video clips are included with the lab settings. | IX-TA-ROAM, , PPG-320 , A-RM-220 , ROAM-B2A , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-23: Lie Detection and Facial RecognitionOur eyes usually give us away when we lie due to their movement. Regular eye movement under questioning usually means we are being truthful, but if that movement changes suddenly and drastically then it’s probably going to be due to a lie being told. This is what an eye tracking system looks for when monitoring a person’s eyes during questioning about events that have happened or people they may know or have seen. | IX-TA-ROAM, , EM-220 , ROAM-B2A , A-iTRAX | GoalsOutcomes |
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HP-24: Myth BustersStudents will create a hypothesis and design an experiment around one of these types of alternative therapies or they can find one of their own (ex - crystals). Recordings of heart rate, GSR, EEG, ECG, and/or other parameters can show whether or not these devices work to reduce overall stress. Studies can be either short- or long- term, and a simple t-test can determine statistical validity. | IX-TA-ROAM, , ROAM-B2A , PPG-320 , BP-220 , A-RM-220 , C-GSR-320 | UHK-TR, UAHK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-28: Sports Psychology - Music & EEGStudents will be looking at EEG and heart rate before and while listening to music. It is thought that if the subject is listening to upbeat, motivational music, you will see certain changes in both EEG and heart rate. | IX-TA-ROAM, , ROAM-B2A , PPG-320 , A-HB-ROAM | UHK-TR, UAHK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-29: Attractiveness PhysiologyStudents will look at a series of images, rank the person’s attractiveness (rank 1-4) and record physiological parameters such as GSR, pulse, heart rate, and skin temperature. | IX-TA-ROAM, , C-GSR-320 , PPG-320 , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HP-30: Control of EEG and BiofeedbackThis lab uses EEG, meditation and scoring to see if brain wave activity can be controlled to lessen feeling of anxiety and it also can bring in the challenge of having to compete with another subject. | IX-TA-ROAM, , ROAM-B2A , A-HB-ROAM | UHK-TR, UAHK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
HK-03: Show Osmosis and diffusion in a cell membraneIn this experiment students will be observing the movement of molecules through a semi permeable membrane. We will be able to observe how some molecules (starch) are too large to pass through a membrane, while smaller molecules (iodine) can freely move. | IX-TA-ROAM, , ODC-320 | GoalsOutcomes |
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| Equipment Required | Included with following Kits | Goals and Outcomes | |
HP-11: Experiment BuilderIn this tutorial, learn how to automate your own experiment with Macros. | IX-TA-ROAM, | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | GoalsOutcomes |
HP-15: Hypothesis-driven Prepulse InhibitionThis lab is meant to be a “hypothesis-driven” lab where students will come up with a series of sounds that they would like their subject to listen to order to elicit the subject's Prepulse Inhibition to a startle stimulus. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-16: Eriksen Flanker TestThe classic flanker effect using arrows: Flankers will be arrows which are presented next to target stimuli and which have been shown to interfere with target responses. All arrows may be facing the same direction (congruent), or the center arrow may be facing the opposite direction (incongruent). The classic flanker effect shows differences in reaction times for response to both the congruent and incongruent arrows. | IX-TA-ROAM, , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HP-17: Craik Memory TestCraik Memory Test shows how when something is related to something you already know, it is easier to commit to memory and recall. This lab aims to look at various levels of processing and see which leads to better memory. | IX-TA-ROAM, , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HP-18: Posner Attention TestPosner's spatial cueing task is a timing task used to measure manual and/or eye-movement reaction to target stimuli in order to determine the effects of covert orienting of attention in response to different cue conditions. | IX-TA-ROAM, , ROAM-B2A , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
HP-19: Lexical Decision TaskSubjects will respond “Yes” or “No” when presented a series of stimuli representing words and non-words. Both timing and error rate will be determined to see how the subjects respond best to these stimuli. | IX-TA-ROAM, , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HP-25: Feature Integration TheoryWhen performing a Feature Integration experiment, colored letters are used in different locations on a field. Fast and accurate recognition of the whether the letter if present or not, or if a specific color of letter is present is one way to test this theory. | IX-TA-ROAM, , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
HP-26: Spatial LocationThis experiment asks observers to look for a curved letter (O, D, Q or G) presented in a circular format with distractor letters in place. It is thought that if subjects reported the distractor letters adjacent to the first curved letter, this would indicate that attending to location is involved in the perception of individual features, and not only in their conjunction. | IX-TA-ROAM, , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | GoalsOutcomes |
HP-27: Spontaneous ImageryThe task is to determine, as quickly as possible, whether the arrow is pointing directly at any of the previously seen dots. It has been shown that mental images preserve spatial distances as in the Finke & Pinker Paradigm (1982). In experiments on image scanning, participants had to first memorize visual information shown in a picture and then answer a question about that image. | IX-TA-ROAM, , RPD-320 | UHK-TR, UAHK-TR, PK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
AM-01: Frog SkeletalMuscle-Weight-WorkIn this experiment, you will use a displacement transducer to determine how weight affects the shortening of a frog muscle. Weight influences the time that a muscle has to shorten, the speed at which the muscle shortens, the distance that the muscle shortens or moves the weight, and the amount of work the muscle completes. You will also compare the difference between: afterloading, supporting the weight before contraction; and preloading, hanging the weight on the muscle without support before contraction. | IX-TA-ROAM, , DT-475 , A-BST-100 , A-FEM-7025 , A-STK-125 | Goals1. Students will dissect a frog leg to extract the gastrocnemius muscle of the lower limb. 2. Students will assemble the equipment needed to be able to stimulate the muscle and record muscle twitch. 3. Students will understand the correlation between the stimulus, the muscle twitch amplitude, and the effect of weight on muscle contractions. 4. Students will test afterloading, supporting the weight before contraction; and preloading, hanging the weight on the muscle without support before the contraction. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully excise the gastrocnemius muscle of a frog’s leg. 2. have a better understanding of electrical stimulation of the muscle and the equipment used to perform such stimulation. 3. gain an understanding of muscle stimulation and contraction (twitch) and how they relate to each other. 4. record muscle twitches from the gastrocnemius, test a variety of hypotheses and reach scientific conclusions 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. | |
AM-02: Frog Skeletal Muscle - Summation and TetanusMost skeletal muscles are composed of some combination of the different twitch-type fibers. Interestingly, a motor neuron makes only one synapse on each of their target fibers, and the muscle fibers innervated by a motor neuron are all of the same type. Therefore, stimulation of a particular motor neuron will create a contraction of only one muscle fiber type; this property is used by the brain to recruit different muscle fibers into a contraction. A motor unit is composed of only one type of muscle fiber and recruitment allows the increase in individual motor units. Activity in descending tracts excites the spinal motor neurons; but, the size of the cell bodies and the activation thresholds of these neurons are different. motor neurons that supply weak, slow, oxidative fibers have the lowest threshold; those innervating fast, intermediate-strength oxidative fibers have higher thresholds; and those that supply the fast, strong, glycolytic fibers have the highest thresholds. In this way increasing the amount of activity descending from the brain activates progressively more motor neurons, and more of the stronger muscle fibers, into the response. This will be simulated in the following experiment by slowly increasing the voltage applied directly to the muscle to recruit more muscle fibers into the contraction. In addition, the amount of contraction is dependent upon stimulus frequency. | IX-TA-ROAM, , FT-302 , A-BST-100 , A-FEM-7025 , A-STK-125 | UAHK-TR, | Goals1. Students will dissect a frog leg to extract the gastrocnemius muscle of the lower limb. 2. Students will assemble the equipment needed to be able to stimulate the muscle and record muscle twitch. 3. Students will understand the correlation between the stimulus, muscle twitch, and the strength of the stimulation on muscle contraction. 4. Students will test summation and tetanus by repeatedly stimulating the muscle. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will:
1. be able to successfully excise the gastrocnemius muscle of a frog’s leg.
2. have a better understanding of electrical stimulation of the muscle and the equipment used to perform such stimulation.
3. gain an understanding of muscle stimulation and contraction (twitch) and how they relate to each other.
4. successfully record muscle twitches from the gastrocnemius and correlate the reactions to stimulation to summation and tetanus.
5. have used the functions available in the Analysis window to determine values necessary for this exercise.
6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-03: Frog Heart MuscleIn this laboratory exercise, students will use a force transducer to monitor the contractility of the frog heart as it is subjected to various imposed conditions, such as: the effect of adding Epinephrine and Acetylcholine to change the heart rate of the exposed heart; the effect of cold temperature on cardiac muscle activity; the effect of electrical stimulation on the refractory period of the heart; and, the effect of interrupting the conduction path between the atria and the AV node with a ligature. | IX-TA-ROAM, , FT-302 , A-BST-100 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a frog to expose the heart. 2. Students will assemble the equipment needed to be able to stimulate the heart and will record cardiac contractions. 3. Students will understand the correlation between exogenous stimulus and heart muscle response. 4. Students will gather data corresponding to normal heart rhythms. 5. Students will test different parameters with regard to cardiac muscle function: ◦ cold temperature. ◦ epinephrine. ◦ atropine. ◦ isolation of the ventricle. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate the frog heart. 2. have a better understanding of electrical stimulation of the heart muscle and the equipment used to perform such stimulation. 3. gain an understanding of normal cardiac muscle contraction. 4. stimulate and record cardiac muscle contractions to test a variety of hypotheses and reach scientific conclusions. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-04: Rat Uterine Motility with Displacement TransducerThe purpose of this experiment is to demonstrate some of the contractile properties of smooth muscle using an isolated rat uterus: spontaneous contractile activity, the effect of stretching the muscle, and the effects of various agonists on the frequency and the degree of contraction. | IX-TA-ROAM, , DT-475 , STB-TR , A-TB-MXBLK , A-STK-125 | Goals1. Students will dissect a female rat to excise the uterus to be able to examine smooth muscle contractions. 2. Students will assemble the equipment needed to be able to record smooth muscle contractions. 3. Students will gather data corresponding to normal rhythmic smooth muscle contractions. 4. Students will test different parameters with regard to uterine muscle function: ◦ methergine. ◦ acetylcholine. ◦ atropine. ◦ epinephrine. ◦ stretch and tension. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate the rat uterus. 2. activate and record uterine muscle contractions to test a variety of hypotheses and reach scientific conclusions. 3. gain an understanding of rhythmic smooth muscle contractions. 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. | |
AM-05B: Rat Intestinal Motility with Force TransducerStudents will observe and record spontaneous smooth muscle activity from the rat jejunum or ileum before recording and analyzing the responses of this tissue to physiologically relevant agents or conditions, such as: stretch, acetylcholine (ACH), curare with or without ACH, atropine with or without ACH, epinephrine, serotonin, changes in pH, changes in calcium ion concentration, and sodium cyanide. | IX-TA-ROAM, , FT-302 , STB-TR , A-TB-MXBLK , A-STK-125 | Goals1. Students will dissect a rat to be able to examine smooth muscle contractions. 2. Students will assemble the equipment needed to be able to record smooth muscle contractions. 3. Students will gather data corresponding to normal rhythmic smooth muscle contractions. 4. Students will test different parameters with regard to uterine muscle function: ◦ acetylcholine. ◦ atropine. ◦ epinephrine. ◦ serotonin, ◦ stretch and tension. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to record the spontaneous contractions of the intestine. 2. describe any qualitative differences in the contractions produced by Acetylcholine, Epinephrine and other pharmaceuticals. 3. understand the difference between excitation and inhibition 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. | |
AM-06: Frog ElectrocardiogramIn this laboratory exercise, students will record the effects of temperature on the amplitude and frequency of electrical activity in the frog heart, as well as the effects of the synaptic transmitters, epinephrine and acetylcholine on these same parameters. | IX-TA-ROAM, , IWIRE-B3G , C-ISO-F3 , A-STK-125 | NBK-TR, | Goals1. Students will dissect a frog to expose the heart. 2. Students will assemble the equipment needed to be able to stimulate the heart and will record cardiac contractions to look at an ECG. 3. Students will understand the correlation between external stimuli and heart muscle response. 4. Students will gather data corresponding to normal heart rhythms. 5. Students will test different parameters with regard to cardiac muscle function and ECG recordings: ◦ cold temperature. ◦ warm temperature. ◦ epinephrine. ◦ acetylcholine. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate the frog heart. 2. gain an understanding of normal cardiac muscle contraction and normal amphibian ECG waves. 3. record cardiac muscle ECG waves to test a variety of hypotheses and reach scientific conclusions. 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-07: Crayfish HeartIn this laboratory exercise, students will use a force transducer to monitor the contractility of the crayfish heart as it is subjected to various imposed conditions, such as: the effect of adding Serotonin and GABA to change the heart rate and contractile force of the exposed heart; and the effect of cold temperature on cardiac muscle activity. | IX-TA-ROAM, , FT-302 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a crayfish to expose the heart. 2. Students will assemble the equipment needed to record cardiac contractions. 3. Students will understand the correlation between eternal stimuli and heart muscle response. 4. Students will gather data corresponding to normal heart rhythms. 5. Students will test different parameters with regard to cardiac muscle function: ◦ cold temperature. ◦ serotonin. ◦ GABA. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate the crayfish heart. 2. gain an understanding of normal cardiac muscle contraction. 3. record cardiac muscle contractions to test a variety of hypotheses and reach scientific conclusions. 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-08: Byssal Retractor Muscle in BivalvesIn this experiment, students will record the phasic and tonic responses of a byssal muscle to individual and repeated stimulus pulses, and the effects of the direct application of acetylcholine and serotonin to the muscles. Students will use the anterior byssal retractor muscle (ABRM), which has a dual origin at the anterior point of the valves and an insertion at the base of the foot. The ABRM is an example of a catch muscle, which is able to maintain a steady contraction with very little energy expenditure. This is an important ability to have in anaerobic environments. The ABRM tends to contract tonically by slowly generating force and maintaining the contraction for some time after the excitatory input has ceased. The ABRM is used because it is easily isolated, stimulated, and monitored by using a force transducer. | IX-TA-ROAM, , FT-302 , C-BNC-PN2 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a marine mussel (Mytilus sp.) to expose the byssal retractor muscle. 2. Students will assemble the equipment needed to be able to stimulate the retractor muscle and will record muscle contractions to look at muscle twitch and responses to stimuli. 3. Students will be able to successively increase stimulation to cause changes in response of the muscle. 4. Students will deliver differing doses of neurotransmitters to see the effects on an actively contracting muscle: ◦ acetylcholine. ◦ serotonin. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to determine the relationship between the strength of the stimulus and the response of the muscle. 2. be able to measure the amplitude of contraction produced in a muscle that is stimulated with a long current pulse, and repeated pulses delivered at progressively higher frequencies. 3. understand the relationship between summation and tetanus. 4. observe the effects of acetylcholine and serotonin, the neurotransmitters that effect contraction and relaxation of the anterior byssal retractor muscle. 5. have used the functions available in the Analysis window to determine values necessary for this exercise and feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-09: Crayfish Gut PharmacologyIn this experiment, you will use a force transducer to record the effects of several transmitters on the rate and force of crayfish intestinal contractions. The recordings also reflect how smooth the peristaltic contractions are and indicate the degree of coordination between the longitudinal and circular muscle layers. | IX-TA-ROAM, , FT-302 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a crayfish abdomen to expose the gut and intestines. 2. Students will assemble the equipment needed to be able to dose the intestine with different drugs and will record muscle contractions to observe responses both type and concentration of drug. 3. Students will deliver differing doses of excitatory and inhibitory neurotransmitters to see the effects on an actively contracting muscle: ◦ acetylcholine. ◦ epinephrine. ◦ GABA. 4. Measure and understand contraction amplitudes and durations. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to record the spontaneous contractions of the intestine. 2. describe any qualitative differences in the contractions produced by Acetylcholine, Epinephrine and GABA. 3. understand the difference between excitation and inhibition 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AM-10: Frog-NerveMuscle - Summation, Tetanus, and Fatigue in a Intact Nerve/Muscle PrepThe most convenient way of examining the properties of the motor unit is by use of the nerve-muscle preparation. With an exposed, centrally ligated nerve trunk (e.g. frog sciatic) supplying a muscle group, a stimulator can be used to excite the distal end of the exposed nerve. Activation of part or all of this trunk will cause a contraction in the innervated muscle. However, even when a successfully propagated impulse has been initiated in a nerve fiber, it must be conducted across the neuromuscular junction to be effective in causing a contraction of the muscle. Not only is a finite time occupied in the transmission of the nerve impulse across the junction, but the junction itself is very sensitive to fatigue and to its chemical environment. | IX-TA-ROAM, , FT-302 , C-BNC-PN2 , C-BNC-SE , A-TB-MXBLK , A-STK-125 | Goals1. Students will dissect a frog leg to extract the sciatic nerve and the muscles of the lower limb (either the gastrocnemius or tibialis anterior). 2. Students will assemble the equipment needed to be able to stimulate the nerve and muscles, and record compound action potentials from both. 3. Students will understand the correlation between nerve stimulus and muscle responses. 4. Students will test synaptic delay between nerve and muscle compound action potentials. 5. Students will test stimulus frequency, fatigue, and myoneural blocking. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully excise the sciatic nerve and muscles of a frog’s leg. 2. have a better understanding of electrical stimulation of nerve fibers and the equipment used to perform such stimulation. 3. gain an understanding of both nerve and muscle compound action potentials and how they relate to each other. 4. record compound action potentials from the sciatic nerve and lower limb muscles to test a variety of hypotheses and reach scientific conclusions. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. | |
AM-11: Earthworm Smooth Muscle ContractionsIn this experiment the student will remove the combined crop-gizzard from the animal and place it in a tissue bath at room temperature. Once the tissues are connected to a force transducer, the student can record spontaneous contractions and the effects of neurotransmitters like acetylcholine and serotonin. This experiment has none of the disadvantages of the mammalian exercise. The animals can be purchased from a bait shop for only a few dollars a dozen, kept in the refrigerator until needed, and used at room temperature. Furthermore, since they are invertebrates, no special authorization is required. | IX-TA-ROAM, , FT-302 , STB-TR , A-TB-MXBLK , A-STK-125 | Goals1. Students will dissect an earthworm to be able to examine smooth muscle contractions. 2. Students will assemble the equipment needed to be able to record smooth muscle contractions. 3. Students will gather data corresponding to normal rhythmic smooth muscle contractions. 4. Students will test different parameters with regard to smooth muscle function: ◦ acetylcholine. ◦ serotonin ◦ epinephrine. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate earthworm gut 2. activate and record uterine muscle contractions to test a variety of hypotheses and reach scientific conclusions. 3. gain an understanding of rhythmic smooth muscle contractions. 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. | |
AM-12: Crayfish ElectrocardiogramAs the heart contracts, its electrical activity can be recorded and displayed in an electrocardiogram (ECG). While the crustacean ECG does not have the characteristic components of the vertebrate ECG, a coordinated depolarization is clearly identifiable, and is analogous to the QRS complex of the vertebrate ECG. This is followed by a slower wave corresponding to the heart’s repolarization, a wave that is analogous to the T wave of the vertebrate ECG. The mechanical activity of the crayfish heart can be monitored by a force transducer, and that exercise is available as Experiment AM-5. In this laboratory exercise, students will record the electrical activity of the heart, looking at the effects of temperature on heart rate and on the amplitude of the components of the ECG, which represent the heart’s summed electrical activity. The effects of two modulatory chemicals, serotonin and GABA, are also explored. | IX-TA-ROAM, , IWIRE-B3G , C-ISO-F3 , A-STK-125 | NBK-TR, | Goals1. Students will dissect a crayfish to expose the heart. 2. Students will assemble the equipment needed to be able to stimulate the heart and will record cardiac contractions to look at an ECG. 3. Students will understand the correlation between external stimuli and heart muscle response. 4. Students will gather data corresponding to normal heart rhythms. 5. Students will test different parameters with regard to cardiac muscle function and ECG recordings: ◦ cold temperature. ◦ warm temperature. ◦ epinephrine. ◦ acetylcholine. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully isolate the frog heart. 2. gain an understanding of normal cardiac muscle contraction and normal crayfish ECG waves. 3. record cardiac muscle ECG waves to test a variety of hypotheses and reach scientific conclusions. 4. have used the functions available in the Analysis window to determine values necessary for this exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
AN-01: Membrane Potentials in CrayfishThe aim of this laboratory exercise is to record resting potentials across the membranes of fast extensor muscle fibers in the tail of crayfish. Microelectrodes are glass capillary tubes which have been melted and then pulled to produce a very fine (<0.5 um diameter) tip at one end. The tip is placed through the membrane and is so fine that the membrane seals around the tip. The microelectrode filled with potassium chloride acts as a “saline bridge” between the inside of the cell and the recording equipment. | IX-TA-ROAM, , IC-200 , A-STK-125 | NBK-TR, | Goals1. Students will dissect a crayfish tail to expose the fast extensor muscles. 2. Students will assemble the equipment to record membrane potentials. 3. Students will understand the Na+/K+ pump and how it works to keep membranes polarized for contraction. 4. Students will test the hypothesis that all fibers within a single muscle are the same and therefore have the same membrane potentials. 5. Students will also test the hypothesis that membrane potentials are dependent upon the concentration gradient of different ions. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully expose the muscles of the crayfish tail and be able to distinguish between the different muscle types. 2. have a better understanding of microelectrode recording from muscle fibers and the equipment used to perform such recordings. 3. gain an understanding of the Na+/K+ pump and how it relates to membrane potentials. 4. record membrane potentials from the different crayfish tail muscles to test a hypothesis and reach a scientific conclusion. 5. test various saline solutions to determine if the concentration of K+ ions has any effect on muscle membrane potentials. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
AN-02: Compound Action Potentials using FrogsIn this laboratory you will record action potentials from the sciatic nerve of a frog. Each nerve contains hundreds of axons with different diameters, thresholds, and degrees of myelination. The large, myelinated axons with the fastest conduction velocities are known as Type A fibers, which are further subdivided into α, β, γ, and δ types. Type B fibers are also myelinated, but have smaller diameters and slower conduction velocities. Type C fibers are very small, unmyelinated axons. When a large stimulus is delivered to the nerve, many axons respond and the recorded potential is the summation of all the axons firing. This potential is known as the compound action potential (CAP). | IX-TA-ROAM, , IWIRE-B3G , NBC-501 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a frog leg to extract the sciatic nerve. 2. Students will assemble the equipment needed to be able to stimulate the nerve and record compound action potentials from nerves. 3. Students will understand the different types of fibers that make up the large sciatic nerve. 4. Students will test different hypothesis with regard to nerve function: ◦ Compound action potential: observing the one or more populations of different fiber types. ◦ Stimulus-response/axon recruitment: observing how the nerve response changes with increased stimulus voltage. ◦ Conduction velocity: measuring the speed at which action potentials propagate down the axons. ◦ Effects of temperature: observing how cooling affects the nerve conduction velocity. ◦ Bidirectionality: determining whether axons conduct in both directions. ◦ Refractoriness: observing how stimulus frequency affects the amplitude of compound action potentials ◦ Strength-Duration: observing how the amplitude of a stimulus required to stimulate axons is related to the duration of the stimulus. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully excise the sciatic nerve of a frog and be able to understand the different fiber types within the nerve. 2. have a better understanding of electrical stimulation of nerve fibers and the equipment used to perform such stimulation. 3. gain an understanding of compound action potentials and how they relate to nerve function. 4. record compound action potentials from the sciatic nerve to test a variety of hypotheses and reach scientific conclusions. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AN-03: Neuromuscular Studies in FrogsThe purpose of this experiment is to demonstrate some of the electrical properties of a muscle and its motor neurons. Nerve and muscle action potentials will be recorded from a frog nerve-muscle preparation. The conduction time and synaptic delay of the neuromuscular unit will be determined. The effect of stimulus frequency, as well as the effects of specific chemical agents upon the muscle and nerve and the neuromuscular junction, will also be measured. | IX-TA-ROAM, , IWIRE-B3G , NBC-501 , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will dissect a frog leg to extract the sciatic nerve and the muscles of the lower limb (either the gastrocnemius or tibialis anterior). 2. Students will assemble the equipment needed to be able to stimulate the nerve and muscles, and record compound action potentials from both. 3. Students will understand the correlation between nerve stimulus and muscle responses. 4. Students will test synaptic delay between nerve and muscle compound action potentials. 5. Students will test different drugs with regard to nerve and muscle function: ◦ eserine. ◦ curare. ◦ atropine. ◦ high acetylcholine concentration. ◦ nicotine. ◦ dantrolene. ◦ high magnesium concentration. ◦ high calcium concentration. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully excise the sciatic nerve and muscles of a frog’s leg. 2. have a better understanding of electrical stimulation of nerve fibers and the equipment used to perform such stimulation. 3. gain an understanding of both nerve and muscle compound action potentials and how they relate to each other. 4. record compound action potentials from the sciatic nerve and lower limb muscles to test a variety of hypotheses and reach scientific conclusions. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AN-04: Action Potentials in EarthwormIn this laboratory you will record action potentials from the ventral nerve cord of an annelid, the earthworm Lumbricus terrestris. The ventral nerve cord of some invertebrates is a structure analogous to the dorsal nerve cord of vertebrates. The ventral nerve cord of an earthworm contains three giant neurons. The cord has one medial giant neuron with a lateral giant neuron on either side of the medial neuron. Because of their size, the three giant neurons in the earthworm nerve cord can generate action potentials with conduction velocities that permit the worms to have a fast escape reflex. When a large stimulus is delivered to the nerve cord, the neurons respond and action potentials from the medial and lateral neurons are seen. | IX-TA-ROAM, , NBC-501 , IWIRE-B3G | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will anesthetize an earthworm. 2. Students will assemble the equipment needed to be able to stimulate the nerve and record compound action potentials from nerves. 3. Students will understand the different types of fibers that make up the nerve. 4. Students will test different hypothesis with regard to nerve function: ◦ Compound action potential: observing the one or more populations of different fiber types. ◦ Stimulus-response/axon recruitment: observing how the nerve response changes with increased stimulus voltage. ◦ Conduction velocity: measuring the speed at which action potentials propagate down the axons. ◦ Effects of temperature: observing how cooling affects the nerve conduction velocity. ◦ Bidirectionality: determining whether axons conduct in both directions. ◦ Refractoriness: observing how stimulus frequency affects the amplitude of compound action potentials ◦ Strength-Duration: observing how the amplitude of a stimulus required to stimulate axons is related to the duration of the stimulus. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to successfully anesthetize an earthworm. 2. be able to understand the different fiber types within the nerve and record CAPs from that nerve. 3. have a better understanding of electrical stimulation of nerve fibers and the equipment used to perform such stimulation. 4. gain an understanding of compound action potentials and how they relate to nerve function. 5. test a variety of hypotheses and reach scientific conclusions. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AN-05: Cockroach Leg MechanoreceptorsIn this experiment, students will collect data that can be used to discover how animals (cockroaches) are able to interpret mechanosensory input. The specific neuronal principles that students will explore include: • Phasic vs. tonic sensory responses • Coding of stimulus intensity by frequency of action potentials • Neuronal adaptation • Temperature response | IX-TA-ROAM, , C-ISO-PN , IWIRE-B3G , A-STK-125 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will explore the basic characteristics of the chordotonal organs. 2. Students will record their response to direction and intensity of leg movement, and determine if the responses are tonic or phasic. 3. Students will learn the basic characteristics of tibial spines on the cockroach leg. 4. Students will determine the effect of repeated stimulations on the frequency of action potentials. 5. Students will also determine the effect of cold and warm temperatures on the neuronal response of mechanoreceptors to mechanical stimulation. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the difference between tonic and phasic responses during flexion and extension. 2. determine whether flexion or extension causes a greater response in action potential frequency, number, or response duration. 3. determine if movement of the tibia spine in one direction or the other causes different responses in action potential frequency, number or duration. 4. understand adaptation of responses. 5. be able to explain how and why physiological processes are dependent on temperature in poikilotherms. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AN-07: Cockroach Cercal Sense OrgansIn this experiment, students will collect data that can be used to discover how animals (cockroaches) are able to interpret mechanosensory input. The specific neuronal principles that students will explore include: • Coding of stimulus intensity by frequency of action potentials • Neuronal adaptation • Spatial summation | IX-TA-ROAM, , IWIRE-B3G , C-ISO-H1 , C-BNC-PN2 , A-STK-125 , C-ISO-PN | NBK-TR, | Goals1. Students will assemble the equipment needed to be able to stimulate the cercal sense organs. 2. Students will elicit a ventral nerve cord response to air puffs and become familiar the responses. 3. Students will explore the effects of stimulus intensity on the number and frequency of action potentials produced. 4. Students will determine the number of hairs needed to trigger an action potential in the ventral nerve cord. 5. Students will determine the effect of a continuous stimulus on the frequency of action potentials. 6. Students will look at the response to a single stimulus after fairly complete adaptation. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. will be able to graph the response changes as a function of intensity. 2. understand whether the response is a change as a function of intensity. 3. be able to explain how the response differences would be important to the cockroach in its environment. 4. be able to determine the minimum number of hairs required to elicit a response. 5. understand the concept of adaptation and explain the importance to cockroach survival. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. 7. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
AN-10: Crayfish Stretch ReceptorsStudents will record responses from two different types of stretch receptors that are positioned next to the dorsal superficial extensor muscles in the tail of the crayfish. Each type of sensory organ responds to a different stimulus, generates impulses of a different amplitude and frequency, and adapts to prolonged stimuli at a different rate. | IX-TA-ROAM, , IWIRE-B3G , A-SUCTIONELEC , A-STK-125 | NBK-TR, | GoalsOutcomes |
AN-12A: Crayfish Neuromuscular JunctionThe purpose of this experiment is to demonstrate the effect that electrical activity (action potentials) in a presynaptic cell has on the electrical activity (synaptic potentials) in a postsynaptic cell. In this experiment, the same technique used to record the resting membrane potentials of frog muscle fibers is used to record the synaptic potentials from crayfish muscle fibers. Synaptic potentials will be recorded using the a microelectrode and an intracellular electrometer. At the same time, the same technique used to record compound action potentials from frog nerve is employed to record the action potentials of crayfish motor axons. These spontaneously generated action potentials will be recorded using a suction electrode and an extracellular amplifier. | IX-TA-ROAM, , IWIRE-B3G , IC-200 , A-SUCTIONELEC , A-STK-125 | NBK-TR, | GoalsOutcomes |
AN-12B: Neuromuscular Junction in Crayfish - part BContinuation of Crustacean NMJ-A In this experiment, the same technique used to record the resting membrane potentials of frog muscle fibers is used to record the synaptic potentials from crayfish muscle fibers. Synaptic potentials will be recorded using the a microelectrode and an intracellular electrometer. At the same time, the same technique used to record compound action potentials from frog nerve is employed to record the action potentials of crayfish motor axons. These spontaneously generated action potentials will be recorded using a suction electrode and an extracellular amplifier. | IX-TA-ROAM, , IWIRE-B3G , IC-200 , A-SUCTIONELEC , A-STK-125 , C-BNC-P2 | NBK-TR, | GoalsOutcomes |
AN-13: Crayfish Motor NerveThe purpose of this experiment is to record the extracellular action potentials of crayfish motor axons. These spontaneously generated action potentials will be recorded using a suction electrode. | IX-TA-ROAM, , IWIRE-B3G , A-SUCTIONELEC , A-STK-125 | NBK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
AMe-01-iWireGA: SmallAnimalRER-iWireGAIn this experiment, students will measure the expired CO2 and consumed O2values, RER, and proportion of fat and carbohydrates utilized while an endotherm (mouse or rat) is in the small animal chamber. They will repeat the experiment with an ectothermic organism (frog, snake or lizard) and make a comparison between the RER values of these two types of animals. Students will then be able to make an accurate assessment of the metabolic capability of these organisms. | IX-TA-ROAM, , IWIRE-PEAK , AC-520 , A-1338-ED | Goals1. Students will distinguish between an ectothermic and endothermic animal. 2. Students will assemble the equipment to be able to record accurate gas analysis measurements. 3. Students will be able to place an animal in the small animal chamber. 4. Students will be able to maintain animal health and well being during recording. 5. Students will accurately analyze data to compare RER values between ectotherms and endotherms. 6. An an option, students may record using animals cooled to lower than body temperature and RER will calculated as they return to normal body temperature or to room temperature. OutcomesStudents who have successfully completed this exercise will: 1. determine mean RER of an endotherm at rest. 2. determine the changes in CO2 and O2 volumes of an ectotherm. 3. make comparisons with the values obtained from an endotherm compared to an ectotherm. 4. design optional experiments to compare other values from different animals. | |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
FB-01: OsmoregulationIn this laboratory, you will use a series of dilutions of seawater (with deionized water) to measure the effects of solute concentration on the movement of water into or out of an aquatic worm or slug. You will place a worm in each solution and then measure its weight change every 10 minutes for one hour. | IX-TA-ROAM, , FT-302 | AHK-TRiw, UAHK-TR, NBK-TR, | Goals1. Students will weigh and observe polychaete worms in different marine salinity dilutions. 2. Students will understand the correlation between saline concentration and osmoregulation in marine organisms. 3. Students will determine the iso- hypo- and hyper- tonic environments based on the loss or gain of weight due to osmosis over time. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. Outcomes1. be able to accurately weigh living polychaete worms to determine weight changes due to osmosis. 2. have a better understanding of osmoregulation and survival of marine organisms. 3. understand the processes of osmosis and diffusion as they relate to living organisms. 4. graph the weight changes of the worms in different salinity concentrations over time to be able to visually understand the concepts. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
BI-01: ECG Signal ConditioningThe Electrocardiogram (ECG/EKG) is an electrical signal produced by the heart muscle. It has an amplitude of about 1mV, so a good amplifier is necessary. Electrical noise or electromagnetic interference (EMI), is generated by many common appliances, such as: power lines, lights, computers, cell phones, etc. When the ECG signal is amplified the noise is amplified as well and often swamps the ECG signal. Signal conditioning the ECG signal is necessary to acquire a good quiet ECG. | IX-TA-ROAM, , ROAM-B2A , A-BREADBOARD | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-02: EMG Signal ConditioningThe Electromyogram (EMG) is an electrical signal produced by skeletal muscle. It has an amplitude of about 1mV, so a good amplifier is necessary. Electrical noise or electromagnetic interference (EMI), is generated by many common appliances, such as: power lines, lights, computers, cell phones, etc. When the EMG signal is amplified the noise is amplified as well and often swamps the EMG signal. Signal conditioning the EMG signal is necessary to acquire a good quiet EMG. | IX-TA-ROAM, , ROAM-B2A , A-BREADBOARD | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-03: ECG NoiseThe Electrocardiogram (ECG/EKG) is an electrical signal produced by the heart muscle. It only has an amplitude of about 1mV, so a good amplifier is necessary. Electrical noise or electromagnetic interference (EMI) is generated by many common appliances, such as: power lines, lights, computers, cell phones, etc. When the ECG signal is amplified the noise is amplified as well and often swamps the ECG signal. Signal conditioning the ECG signal is necessary to acquire a good quiet ECG. Record ECG, with noise, identify the frequency of the noise and filter using various filters | IX-TA-ROAM, , ROAM-B2A , A-BREADBOARD | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-04: Frequency Response of FiltersIn this experiment we will look at the frequency response of various filters both analog as well as digital filters. The iWorx Recorder has the ability to output, through the Stimulator, a sine wave at a particular frequency. We will program Labscribe using Macros to change the frequency of the signal and measure the frequency response of the filter. We will look at how the order of a digital filter affects its frequency response. | IX-TA-ROAM, , A-BREADBOARD | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-05: Op-Amp CircuitsIn this experiment we will look at various operational amplifier circuits. The IX-TA recorder has the ability to output, through the Stimulator, waveforms such as a sine wave or a square wave. We will use these signals as an input to various op-amp circuits and observe the output. | IX-TA-ROAM, , A-BREADBOARD | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-06: Arduino ClawControl an Arduino Claw, from your EMG or grip force. | IX-TA-ROAM, , ROAM-B2A , FT-220 , A-CLAW-KIT | UBIK-TR, | GoalsOutcomes |
BI-07: Make your Force Transducer, using a strain guage and an instrumentation amplifierMake your Force Transducer, using a strain guage and an instrumentation amplifier. The main objective in this experiment is to correlate the change in voltage with the change in strain so that we can pinpoint a certain voltage change as indicative of a certain amount of force applied. | IX-TA-ROAM, , A-BREADBOARD , A-BYFT-Kit | UBIK-TR, | GoalsOutcomes |
BI-08: Multipoint Calibration of the Temperature SensorFor multi-point calibration, collect reference measurements at different points across the sensor’s range. Then, create a calibration curve or equation that relates the sensor’s raw output to the actual value. Use this curve to adjust the subsequent sensor readings. | IX-TA-ROAM, , TM-220 | BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-09: Helmet Impact Testing using the ROAM wirelessThis lab looks at testing impact forces on various helmets to look at safety features. | IX-TA-ROAM, , ROAM-B2A | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
BI-10: EMG-Triggered-Stimulation using the ROAM wirelessIn lab, since we do not have the capability to get Iron Man’s suit or 3-D print a prosthetic, we can use one person as the “brain” and one person as the “prosthesis”. This means that one person will have the ability to actually control the movements of the other! | IX-TA-ROAM, , ROAM-B2A , FT-220 , C-HVS-SL2 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | GoalsOutcomes |
BI-10: EMG-Triggered-Stimulation using the iWire-B3GIn lab, since we do not have the capability to get Iron Man’s suit or 3-D print a prosthetic, we can use one person as the “brain” and one person as the “prosthesis”. This means that one person will have the ability to actually control the movements of the other! | IX-TA-ROAM, , IWIRE-B3G , FT-220 , C-HVS-SL2 | AHK-TRiw, UAHK-TR, NBK-TR, | GoalsOutcomes |
BI-11: Comparing the period with varying pendulum length, mass and angle of displacement.This lab compares the period of oscillation of a pendulum against differing lengths, masses and angles of displacement. | IX-TA-ROAM, , FT-5K , LIS-100 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | GoalsOutcomes |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
CM-01: Oxygen Consumption and SizeThe purpose of this exercise is to measure the rate of oxygen consumption in goldfish of different weights. Studies have shown that the rate of oxygen consumption (moles O2 consumed/unit time) is directly proportional to weight of the organism, meaning larger animals consume more oxygen. However, if metabolic data is expressed as the rate of oxygen consumption per unit weight (moles O2 consumed/unit time/unit weight), the opposite trend is found. Smaller animals consume more oxygen per gram of body weight than larger animals do. A log-log plot of O2 consumption rate/unit body weight against body weight reveals a linear relationship with a slope of around 0.75. | IX-TA-ROAM, , DO2E-200 | Goals1. Students will learn to accurately weigh small organisms. 2. Students will learn to calibrate the dissolved oxygen sensor and measure the rate of oxygen consumption over time of different sized organisms. 3. Students will collect and analyze oxygen consumption curves to determine how oxygen consumption is related to the size of an organism. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have accurately measured the weight of small animals. 2. have successfully calibrated the dissolved oxygen sensor and recorded the oxygen consumption over time of various sized organisms. 3. after analyzing the data collected, be able to relate oxygen consumption to size. 4. come to a conclusion about any trends shown by this experiment. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
CM-02: Mitochondrial MetabolismIn this experiment, you will examine one step within the Krebs cycle: the oxidation of succinic acid to fumaric acid. This reaction is catalyzed by the mitochondrial enzyme succinic dehydrogenase (SDH). SDH is covalently bonded to flavin adenine dinucleotide (FAD). FAD is reduced as succinic acid is oxidized. The reduced FAD passes its electrons through the electron transport system, where they are eventually passed to molecular oxygen to form water. | IX-TA-ROAM, | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | Goals1. Students will examine one step in the process of the Kreb’s Cycle of Cellular Respiration, the oxidation of succinic acid to fumaric acid. 2. Students will use a spectrophotometer to observe changes in the color of dye-labeled mouse liver extract in order to examine rate of reaction. 3. Students will perform three (3) separate experiments: one without cyanide, one in the presence of cyanide, and one using a competitive inhibitor to respiration. 4. Students will collect data, and use linear regression analysis to find the line of best fit for each set of reactions. 5. Students will make a histogram to compare the rate of reaction of color change of the three experiments. OutcomesStudents who have successfully completed this exercise will: 1. understand the process of Cellular Respiration at the mitochondrial level. 2. be able to successfully use a spectrophotometer to measure color changes over time. 3. understand the concept of competitive inhibition. 4. be able to explain what cyanide does to the rate of a cellular respiration reaction. 5. analyze data and design a histogram for data comparison. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. |
CM-03: Mitochondrial RespirationIn this experiment, students will learn how to calibrate and use an oxygen polarograph, how various substrates, inhibitors, and uncouplers affect the rate of electron transport by monitoring changes in the rate of oxygen consumption. This lab will also look at how various electron donors affect P:O ratios. P:O ratios indicate the amount of ATP produced and the number of protons moved across the inner mitochondrial membrane for the number of electrons flowing through the electron transport chain and the amount of oxygen consumed. | IX-TA-ROAM, , DO2E-200 , RPC-100 | Goals1. Students will examine the electron transport process of mitochondrial respiration. 2. Students will use a dissolved oxygen electrode and cellular respiration chamber to observe changes in the amount of dissolved oxygen in a solution of mitochondria order to examine rate of reaction. 3. Students will perform experiments using couplers, uncouplers, inhibitors and donors to see the effects on cellular respiration. 4. Students will collect and analyze data to determine the effects of various chemicals on the respiration process. OutcomesStudents who have successfully completed this exercise will: 1. understand the process of Cellular Respiration at the mitochondrial level. 2. be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time. 3. understand the concept of competitive inhibition how donors, coupler and uncouplers work within the cellular metabolism process. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
CM-04: PhotosynthesisIn this experiment, students will learn how to calibrate and use an oxygen polarograph, how to measure the functionality of isolated thylakoids, how to measure electron transport in a complete photosystem and how to measure electron transport in a single photosystem (PS I). | IX-TA-ROAM, , DO2E-200 , RPC-100 | Goals1. Students will examine the process of photosynthesis using isolated thylakoids from chloroplasts. 2. Students will use a dissolved oxygen electrode and photosynthesis chamber to observe changes in the amount of dissolved oxygen in a thylakoid solution in order to examine rate of reaction. 3. Students will learn how to measure the functionality of isolated thylakoids and how to measure electron transport in a complete photosystem. 4. Students will also learn how to measure electron transport in a single photosystem (PS I). 5. Students will collect and analyze data to determine the effects of various chemicals on the photosynthetic process. 6. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the process of Photosynthesis at the level of chloroplasts and photosystems within isolated plant organelles. 2. be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time. 3. understand how uncouplers affect oxygen production rates in terms of phosphorylation, electron transport and chemiosmosis. 4. understand the relationship between light intensity and the rate of oxygen production in the whole electron transport process and in a single photosystem. 5. be able to compare coupled and uncoupled reactions between different experiments. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
CM-05: CO2 FixationThis experiment also contains three exercises that measure photosynthetic electron transport in thylakoids with the same three compounds. This permits the results from intact cells to be compared to the results obtained from thylakoids that do not have the enzymes of the Calvin Cycle. | IX-TA-ROAM, , DO2E-200 , RPC-100 | Goals1. Students will examine the process of carbon dioxide fixation using intact algal cells. 2. Students will use a dissolved oxygen electrode and photosynthesis chamber to observe changes in the amount of dissolved oxygen in a thylakoid solution in order to examine rate of reaction. 3. Students will use three compounds to compare the effect on the rate of carbon dioxide fixation in intact cells: ◦ Iodoacetamide (IAA), which inhibits certain enzymes of the Calvin cycle, but should have no effect on photosynthetic electron transport. ◦ 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), which blocks electron transport between the Qa and Qb quinones in the chain. ◦ Methylamine (MA), which should increase the rate of electron transport by uncoupling ATP synthesis from electron transport. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the process of Photosynthesis and CO2 fixation in intact algal cells. 2. be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time. 3. understand how uncouplers affect oxygen production rates in terms of phosphorylation, electron transport and chemiosmosis. 4. understand the relationship between CO2 fixation in intact algal cells in both the dark and light; and the rate of oxygen production during the Calvin Cycle. 5. be able to compare oxygen production in algal cells and isolated thylakoids using the different chemicals. 6. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 7. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
CM-06: Whole Pant Gas AnalysisIn this lab students will use a gas analyzer to observe the exchange of CO2/O2 and calculate the gas exchange ratio of a photosynthesizing plant. Students will also be able to adjust starting carbon dioxide concentrations, alter temperature or light availability to see the effect these change have on CO2 utilization and O2 production during photosynthesis. | IX-TA-ROAM, , IWIRE-PEAK , AC-520 | Goals1. Students will assemble the equipment to be able to record accurate gas analysis measurements. 2. Students will determine the rate of oxygen production and carbon dioxide utilization in a photosynthesizing organism. 3. Students will accurately analyze REE values. 4. An an option, students may record: ◦ A plant a room temperature and compare to a plant at either high or low temperature. ◦ A plant contained in one concentration of CO2 compared to a plant in a higher concentration of CO2. ◦ A plant with the light shining on the leaves to a plant in the dark. OutcomesStudents who have successfully completed this exercise will: 1. determine mean REE of a plant. 2. determine the changes in CO2 and O2 concentrations over time. 3. make comparisons with the values obtained under different circumstances. 4. Use advanced analysis features to gather mathematical data. | |
CM-07: Oxygen Consumption and Aerobic RespirationIn most metazoans metabolism is supported by aerobic production of energy equivalents (ATP). This process harnesses the energy released from the oxidation of glucose (or other substrates). The oxygen consumed is directly proportional to the energy produced (which in turn is proportional to the energy consumed) by the organism. Therefore, measurement of oxygen consumption is a viable indicator of total energy usage by organisms under most circumstances. The rate of oxygen consumption by organisms depends critically on a number of factors. The following must be carefully controlled: temperature, oxygen concentration, activity levels, feeding history, and body size. In the present experiment, we will examine the effects of starvation and temperature on the oxygen consumption rate of goldfish. | IX-TA-ROAM, , DO2E-200 | Goals1. Students will learn to accurately weigh small organisms. 2. Students will learn to calibrate the dissolved oxygen sensor and measure the rate of oxygen consumption over time of organisms under different metabolic conditions. 3. Students will collect and analyze oxygen consumption curves to determine how oxygen consumption is related to the diet or ambient temperature of an organism. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have accurately measured the weight of small animals. 2. have successfully calibrated the dissolved oxygen sensor and recorded the oxygen consumption over time of organisms under various conditions. 3. after analyzing the data collected, be able to relate oxygen consumption to diet or ambient temperature. 4. come to a conclusion about any trends shown by this experiment. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
| Equipment Required | Included with following Kits | Goals and Outcomes | |
GB-01: Biological BuffersIn this experiment, students will determine the buffering capabilities of a variety of solutions by measuring the pH of the solutions when they are treated with either a weak acid or a weak base. Each group of students will measure the pH changes that occur in deionized (DI) water, a buffered physiological saline, and another solution from a list provided. | IX-TA-ROAM, , ISE-100 | Goals1. Students will determine the buffering capabilities of a variety of solutions by measuring the pH of the solutions 2. Students will compare buffering capabilities when the solutions are treated with either a weak acid or a weak base. 3. Students will measure the pH changes that occur in deionized (DI) water, a buffered physiological saline, and another solution chosen from a list provided. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the concept of pH and biological buffers. 2. understand the importance of buffering capabilities in biological systems. 3. be able to mathematically calculate percent change in pH and relate this to the addition of a weak acid or base to a buffered solution. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-02: Membrane PermeabilityIn this experiment, students will examine some of the properties of passive transport mechanisms across a simulated membrane created with dialysis tubing. Each sac created with dialysis tubing will be filled with one of three different solutions, each containing a different set of ions. When each dialysis sac is placed in a beaker of deionized water, ions will move across the membrane. The movement of ions between the dialysis sac and the surrounding water will be indicated by a change in the pH of the water surrounding the simulated cell. | IX-TA-ROAM, , ISE-100 , ODC-320 | Goals1. Students will examine some of the properties of passive transport mechanisms across a simulated membrane. 2. Students will learn to create a “cell” using dialysis tubing. 3. Students will fill the “cells” with different solutions to understand the movement of ions across a cell membrane. 4. Students will measure the change in pH of the water surrounding the simulated cell. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the concept of passive transport and the movement of ions across a cell membrane. 2. be able to explain how the movement of large particles causes a change in pH. 3. be able to understand the rate of diffusion of ions across the membrane. 4. be able to explain the factors that could increase the rate of diffusion of an ion across a membrane. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-02: Membrane PermeabilityIn this experiment, students will examine some of the properties of passive transport mechanisms across a simulated membrane created with dialysis tubing. Each sac created with dialysis tubing will be filled with one of three different solutions, each containing a different set of ions. When each dialysis sac is placed in a beaker of deionized water, ions will move across the membrane. The movement of ions between the dialysis sac and the surrounding water will be indicated by a change in the pH of the water surrounding the simulated cell. | IX-TA-ROAM, , ISE-100 , ODC-320 | Goals1. Students will examine some of the properties of passive transport mechanisms across a simulated membrane. 2. Students will learn to create a “cell” using dialysis tubing. 3. Students will fill the “cells” with different solutions to understand the movement of ions across a cell membrane. 4. Students will measure the change in pH of the water surrounding the simulated cell. 5. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the concept of passive transport and the movement of ions across a cell membrane. 2. be able to explain how the movement of large particles causes a change in pH. 3. be able to understand the rate of diffusion of ions across the membrane. 4. be able to explain the factors that could increase the rate of diffusion of an ion across a membrane. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-03: Water QualityIn this experiment, students will collect water samples from a variety of sources, like streams and ponds, around their community. They can also collect samples of tap water, water from swimming pools and drinking fountains, and deionized or distilled water for testing. Students will measure the temperature of the water at the site where it is collected. When the water samples are brought back to the laboratory, the students will measure the pH, dissolved oxygen concentration, and specific gravity of their samples. The values of these four parameters for each sample will be entered into a table for comparison. | IX-TA-ROAM, , ISE-100 , DO2E-200 | Goals1. Students will collect water samples from a variety of sources, like streams and ponds, around their community. 2. Students will measure the temperature of the water at the site where it is collected. 3. Students will measure the pH, dissolved oxygen concentration, and specific gravity of water samples. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to collect water samples from a wide variety of sources. 2. be able to successfully use pH and DO2 electrodes to measure these parameters in a variety of water samples. 3. understand how pH, dissolved O2 and specific gravity impact water quality. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-04: Ecological BalanceIn this experiment, students will create an environment into which a small aquatic animal, a goldfish, is introduced. Students will measure the changes in the dissolved oxygen concentration and the pH level of the water in which the fish is respiring. Students will also create an environment into which a piece of aquatic plant is introduced. Students will measure the changes in the dissolved oxygen concentration and the pH level of the water when the plant is exposed to light and photosynthesis takes place. In the third exercise, the goldfish is reintroduced into the environment containing the plant, and the changes in the dissolved oxygen concentration and pH level of the ecosystem are measured. It is also determined if this ecosystem is more or less balanced than the other two environments. | IX-TA-ROAM, , ISE-100 , DO2E-200 | Goals1. Students will create an environment into which a small aquatic animal, a goldfish, is introduced. 2. Students will measure the changes in the dissolved oxygen concentration and the pH level of the water in which the fish is respiring. 3. Students will also create an environment into which a piece of aquatic plant is introduced. 4. Students will measure the changes in the dissolved oxygen concentration and the pH level of the water when the plant is exposed to light and photosynthesis takes place. 5. Students will create an environment in which both the plant and fish are present. 6. Students will measure the changes in the dissolved oxygen concentration and pH level of the ecosystem will be measured. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. be able to create specific environments in which to measure pH and dissolved oxygen concentrations. 2. be able to successfully use pH and DO2 electrodes to measure these parameters in three ecological environments. 3. understand how pH, dissolved O2 impact the quality of an ecosystem and be able to determine which biological system is more balanced. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-05: Acid RainIn this experiment, students will generate the gases that create acid rain: carbon dioxide, nitrogen dioxide, and sulfur dioxide. The gases will then be bubbled through water as the acidity of the water is monitored using a pH electrode attached to an iWorx data acquisition unit. | IX-TA-ROAM, , ISE-100 | Goals1. Students will generate the gases that create acid rain: carbon dioxide, nitrogen dioxide, and sulfur dioxide. 2. Students will bubble the gases through water. 3. Students will monitor the acidity of the water using a pH electrode. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand the concept of pH and acid rain. 2. understand the importance of regulating pH in biological systems. 3. be able to mathematically calculate percent change in pH and relate this to the addition of an acid rain producing gases to water. 4. explain why acid rain is deleterious to ecosystems and habitats. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-06: Conductivity of SolutionsIn this experiment, students will test the conductivity of a variety of strong, weak, and non-electrolyte solutions using a conductivity meter attached to an iWorx data acquisition unit. Various electrolyte concentrations will also be studied to see how concentration affects conductivity. Additional exercises may include relating conductivity to temperature, measuring the conductivity of water samples from a variety of sources, and correlating conductivity to pH. | IX-TA-ROAM, , CM-100 | Goals1. Students will learn to use a conductivity meter. 2. Students will calibrate the conductivity meter using standard solutions. 3. Students will test the conductivity of various electrolytes. 4. Students will study the effect of concentration on the conductivity of solutions. 5. Students will graph the concentration vs. conductivity of the various solutions tested. OutcomesStudents who have successfully completed this exercise will: 1. determine the conductivity of different electrolyte solutions. 2. understand the effect of concentration on the conductivity of various solutions. 3. understand the main contributor to the difference in conductivity values between the solutions tested. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values necessary for this exercise. | |
GB-07: Venus Fly Trap ReactionsThe Venus Flytrap does not have a nervous system or any muscles or tendons. Scientists theorize that the traps move from fluid pressure activated by an actual electrical current that runs through each lobe. Using recording electrodes, measuring the action potential of the trap is possible. This can then be compared to human nerve conduction, earthworm, and frog action potentials to see if there are any similarities or differences between species. | IX-TA-ROAM, , ROAM-B2A , C-MS-VFT | GoalsOutcomes |
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| Equipment Required | Included with following Kits | Goals and Outcomes | |
FL-01: Auditory and Visual ReflexesThis lab looks at the physiology behind whether sight or sound stimuli cause a faster reaction time. | IX-TA-ROAM, , EM-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, NBK-TR, | Goals1. Students will gain an understanding of a reflex arc and how the spinal cord and peripheral nerves function in the human body 2. Students will be able to successfully record responses from subjects to auditory and visual stimuli. 3. Students should be able to measure the response time of their subjects to different cues and relate it to the functioning of the spinal nerves. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to draw a reflex arc. 2. have recorded responses of subjects to both auditory and visual stimuli. 3. determine a subject’s response time to various cues. 4. be able to determine the effect of different types of auditory cues on response time. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
FL-02: Exercise, the ECG and Peripheral Circulation (ROAM)You will look at an ECG and heart rate, and determine the effects of moderate exercise | IX-TA-ROAM, , ROAM-B2A , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and examine the relationship between the ECG and the peripheral circulation. 2. Students will be able to record and look at the effects of exercise on an ECG and pulse in different subjects during the lab period. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and the amplitudes of various ECG waves. 6. have been able to examine and interpret the effects of exercise on ECG and pulse amplitudes and timing. |
FL-02: Exercise, the ECG and Peripheral Circulation (iWireB3G)You will look at an ECG and heart rate, and determine the effects of moderate exercise | IX-TA-ROAM, , IWIRE-B3G , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record a three-lead Electrocardiogram (ECG) and examine the relationship between the ECG and the peripheral circulation. 2. Students will be able to record and look at the effects of exercise on an ECG and pulse in different subjects during the lab period. 3. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable ECG. 2. have been able to interpret an ECG, especially the individual P and T waves, and the QRS complex. 3. be able to calculate the heart rate of an individual from the recorded data. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 5. have used the functions available in the Analysis window to determine values for arterial pulse amplitude and heart rate, and the amplitudes of various ECG waves. 6. have been able to examine and interpret the effects of exercise on ECG and pulse amplitudes and timing. |
FL-03: Grip Strength and the Electromyogram (ROAM)You will record EMG activtity in the forearm muscles, You will be able to determine recruitment and fatigue in relation to muscle force. | IX-TA-ROAM, , ROAM-B2A , FT-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will successfully record electromyograms (EMGs). 2. Students will learn how to calibrate a dynamometer and convert pounds to kilograms. 3. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 4. Students should be able to measure the EMG produced and corresponding muscle force. 5. Students will measure the force produced by the muscle in the dominant forearm. 6. Students will also study and measure the effect of fatigue on the muscles in the dominant and non-dominant forearms. Comparison of the measurement will also be examined. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between nerve impulses and the resulting EMG recording. 4. have gained understanding of the responses in the dominant forearm, and the correlation between fatigue and muscle strength. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
FL-03: Grip Strength and the Electromyogram (iWireB3G)You will record EMG activtity in the forearm muscles, You will be able to determine recruitment and fatigue in relation to muscle force. | IX-TA-ROAM, , IWIRE-B3G , FT-220 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, | Goals1. Students will successfully record electromyograms (EMGs). 2. Students will learn how to calibrate a dynamometer and convert pounds to kilograms. 3. Students will gain an understanding of the relationship between the electric current from the nerves and the response of the muscle or muscle group being innervated. 4. Students should be able to measure the EMG produced and corresponding muscle force. 5. Students will measure the force produced by the muscle in the dominant forearm. 6. Students will also study and measure the effect of fatigue on the muscles in the dominant and non-dominant forearms. Comparison of the measurement will also be examined. 7. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. understand and be able to record an EMG. 2. understand how nerves send electrical signals to muscles to cause a response. 3. be able to determine the relationship between nerve impulses and the resulting EMG recording. 4. have gained understanding of the responses in the dominant forearm, and the correlation between fatigue and muscle strength. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
FL-04: Breathing Parameters at Rest and After ExerciseThis lab looks at lung volumes before and after moderate exercise. | IX-TA-ROAM, , A-FH-300 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, UBIK-TR, | Goals1. Students will be able to successfully record respiratory cycles. 2. Students should be able to measure respiration volumes including: tidal volume, reserve capacities, vital capacity, and be able to calculate overall lung volume. 3. Students will be able to determine the difference in lung volumes of a subject at rest, immediately after exercise, and up to a few minutes after exercise. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have recorded a recognizable respiratory cycle at rest. 2. have recorded recognizable respiratory cycles on an individual immediately after exercise and a few minutes after exercise. 3. be able to determine the respiratory volumes of an individual from the recorded data and understand the effects of exercise on lung volumes. 4. determine a subject’s overall fitness and lung health after examining breathing rate recovery from exercise. 5. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. 6. have used the functions available in the Analysis window to determine values necessary for this exercise. |
FL-05: Blood Pressure, Peripheral Circulation and Body PositionIn this lab, students will collect blood pressure looking at various positions of the body | IX-TA-ROAM, , BP-220 , PPG-320 | HK-TR, UHK-TR, AHK-TRiw, UAHK-TR, HEK-TR, BIK-TR, UBIK-TR, PK-TR, | Goals1. Students will be able to successfully record pulse waves using a plethysmograph, and blood pressure using a non-invasive blood pressure cuff (sphygmomanometer). 2. Students will be able to interpret data from these recordings and understand the difference between systolic and diastolic blood pressure. 3. Students will look at the effects of different cuff and body positions on pulse and blood pressure. 4. Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. OutcomesStudents who have successfully completed this exercise will: 1. have successfully calibrated a non-invasive blood pressure cuff. 2. have recorded recognizable pulse and blood pressure waves and be able to calculate the pulse rate and blood pressure of an individual from the recorded data. 3. have been able to interpret the effects of different cuff and body positions on both pulse and blood pressure. 4. feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings. |
Customer Testimonials
"The iWorx system has been a centerpiece in the physiology labs at Boston College for many years. The instruction manual for students is clearly and concisely presented. The technical support is top notch and incredibly helpful. The myriad of options for exploration in each body system is incredible and always being updated, but most importantly, the students come to lab eager to begin and learn something new about their own bodies. They love it!"
Lynn DiBenedetto, MEd, PhDBoston College
"We've been using our iWorx this term, very extensively (three different lab courses for a total of 5 different lab exercises, approximately 500 students have used them this term, some multiple times). and I have to say I'm VERY satisfied with how it has worked. The equipment does what is expected, students have been able to set up, run experiments and tear down straight from the instructions without having to "hold their hands", and my TA's are enjoying labs again (funny how equipment that "works" make's labs more enjoyable!). "
Dr. Bruce Wolff University of Waterloo
"I would like to mention your incredible customer support. We can keep our software updated using your web site. You and other people at iWorx are always available to help with anything, even during Christmas break! You act like there is no such thing as a stupid question."
Dr. Robert WestNorthern Essex Community College
"The iWorx kits are perfect for helping students learn physiology in an applied laboratory setting. As an instructor I appreciate the versatility of the system, which allows me to move from nerve conduction, to cardiology, to digestive physiology, and more. And the students find the system and software easy to use, and are able to generate reliable data with very little training. Overall, I've been extremely satisfied with iWorx and look forward to working with them in the future."
Dr. Brad RabquerAlbion College
"My new iWorx equipment is very student friendly. The software is very understandable and straightforward with most students being able to use it the first week unassisted. The experiments are well laid out including necessary background. I especially like the many fail safe aspects to the equipment. This prevents student injury and potential equipment damage."
Debra Mullikin-Kilpatrick, Ph.D.Boston College
"The combination of hardware and software offered by iWorx, coupled with the manual of experiments that have been tailored to the system, have done the job effectively for us. Setup is fast and easy (...) Data collection and analysis are straightforward with the LabScribe software. Technical support has been both quick and effective."
James M. Mullins, ProfessorThe Catholic University of America
"Just wanted to let you know how helpful the training was on Tuesday. After your explanations I'm sufficiently confident to introduce my students to the process and software in class today. Great class, outstanding equipment!"
Eric Winters, Ph.D.Denison University Athletics Dept.
"Students have found the systems easy to calibrate and reliable. I have found the amplifiers nearly indestructible. The extensive array of parameters that can be evaluated with the systems affords greater opportunities for students to ask mechanistic questions and yet still propose projects of their own design."
Laura Malloy Ph.D.Hartwick College
"I did my first full lab with the equipment yesterday with fabulous results. Physiology Lab at Louisiana College is going to be so much fun this semester. Thanks for making such a great system."
Wade Warren, Ph.D.Louisiana College
"I must say the customer support for iWorx has been super, and it's a pleasure working with you."
Robin Mockett, Ph.D.University of South Alabama
"I really value the flexibility in the iWorx protocols that allows students to engage in hypothesis-driven investigation. They were thrilled to watch and record the resulting nystagmus. It is very gratifying to see my students get excited about learning!"
Tracy Schatteman, Ph.D.University of Illinois
Textbooks using iWorx
- Human Physiology: Lab Manual and Study Guide
Author(s): Patricia Clark - Human Biology Laboratory Manual: BIOL 1140: University of Iowa
- Modern Methods Of teaching BiologyBy Richa Bhatt
- Nervous Systems and Control of BehaviorBy Martin Thiel · 2014

