Isolated tissue and organ preparations allow researchers to investigate the physiology and pharmacology of various tissue samples in a controlled environment without the complications of an intact animal model. These in vitro experiments can be performed using a variety of tissues and organs including smooth muscle, skeletal muscle, cardiac muscle, gastrointestinal and urogenital tissue samples and arterial rings. Typically, these studies are performed in temperature controlled environments with tissues perfused in an oxygenated physiological buffer solution. Typical evoked-response protocols can be carried out using pharmacological agents and/or electrical stimulation.
iWorx Recorders and software works with various tissue bath systems used to conduct isolated tissue and vascular ring studies from a variety of species. We also work with Living Systems, single channel or 4-channel Myograph system for experiments utilizing small rings samples with sizes ranging from 60 mm to over 1 mm in diameter.
MYO-CH Halpern/Mulvaney Style Wire Myograph Chamber
The wire myograph include three types of tissue supports: wire-mounts for microvessels, L-bars for large ring preparations, and a hook for working with strips, making it suitable for a range of applications including force measurements in microvessel rings, large vessels such as carotid artery and aorta, airway, intestine, bladder, and many more.
MYO-SC-1 Force Transducer Signal Conditioner
The MYO-SC-1 Force Transducer Signal Conditioner is designed to convert the force measurement made by the force transducer in our MYO-CH wire myograph chamber into an analog voltage suitable for recording with an analog-to-digital converter or chart recording device.
MYO-01 Halpern/Mulvaney Style Wire Myograph Package
The MYO-01 includes a MYO-CH Wire Myograph Chamber with Modular Tissue Supports and a MYO-SC-1 Force Transducer Signal Conditioner.
MYO-01-SH Self Heated Wire Myograph Package
In some cases, such as when using expensive peptides or other drugs, bath superfusion is not an option. These applications call for minimizing the volume of bath fluid to keep the amount of reagents used to a minimum. In these cases, heating the bath to physiological temperatures can be problematic. However, with the wire myograph chamber (MYO-CH) and temperature controller (TC-09S), you can heat the wire myograph chamber directly to physiological temperatures. This package contains everything needed to conduct these self-heating studies in the wire myograph chamber.