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Cellular Metabolism

Included with:
Lab Equipment Required: IX-TA, ISE-730
Description: Goldfish or other small fish. Calculation of metabolism by looking at the consumption of O2 based on organism size.

Goals

1.	Students will learn to accurately weigh small organisms.
2.	Students will learn to calibrate the dissolved oxygen sensor and measure the rate of oxygen consumption over time of different sized organisms. 
3.	Students will collect and analyze oxygen consumption curves to determine how oxygen consumption is related to the size of an organism. 
4.	Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. 

Outcomes

Students who have successfully completed this exercise will:
1.	have accurately measured the weight of small animals.
2.	have successfully calibrated the dissolved oxygen sensor and recorded the oxygen consumption over time of various sized organisms. 
3.	after analyzing the data collected, be able to relate oxygen consumption to size.
4.	come to a conclusion about any trends shown by this experiment.
5.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
6.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Included with:
Lab Equipment Required:
Description: Uses mitochondria from liver and a spectrophotometer.

Goals

1.	Students will examine one step in the process of the Kreb’s Cycle of Cellular Respiration, the oxidation of succinic acid to fumaric acid. 
2.	Students will use a spectrophotometer to observe changes in the color of dye-labeled mouse liver extract in order to examine rate of reaction. 
3.	Students will perform three (3) separate experiments: one without cyanide, one in the presence of cyanide, and one using a competitive inhibitor to respiration. 
4.	Students will collect data, and use linear regression analysis to find the line of best fit for each set of reactions.
5.	Students will make a histogram to compare the rate of reaction of color change of the three experiments.

Outcomes

Students who have successfully completed this exercise will:
1.	understand the process of Cellular Respiration at the mitochondrial level.
2.	be able to successfully use a spectrophotometer to measure color changes over time.
3.	understand the concept of competitive inhibition.
4.	be able to explain what cyanide does to the rate of a cellular respiration reaction.
5.	analyze data and design a histogram for data comparison.
6.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
7.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Included with:
Lab Equipment Required: IX-TA, ISE-730 , RPC-100
Description: Mouse liver mitochondria for looking at cellular respiration under a variety of experimental conditions.

Goals

1.	Students will examine the electron transport process of mitochondrial respiration.
2.	Students will use a dissolved oxygen electrode and cellular respiration chamber to observe changes in the amount of dissolved oxygen in a solution of mitochondria order to examine rate of reaction. 
3.	Students will perform experiments using couplers, uncouplers, inhibitors and donors to see the effects on cellular respiration.
4.	Students will collect and analyze data to determine the effects of various chemicals on the respiration process.

Outcomes

Students who have successfully completed this exercise will:
1.	understand the process of Cellular Respiration at the mitochondrial level.
2.	be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time.
3.	understand the concept of competitive inhibition how donors, coupler and uncouplers work within the cellular metabolism process. 
4.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
5.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Included with:
Lab Equipment Required: IX-TA, ISE-730 , RPC-100
Description: Photoreceptors from thylakoids – looking at photosynthesis under a variety of experimental conditions.

Goals

1.	Students will examine the process of photosynthesis using isolated thylakoids from chloroplasts.
2.	Students will use a dissolved oxygen electrode and photosynthesis chamber to observe changes in the amount of dissolved oxygen in a thylakoid solution in order to examine rate of reaction. 
3.	Students will learn how to measure the functionality of isolated thylakoids and how to measure electron transport in a complete photosystem. 
4.	Students will also learn how to measure electron transport in a single photosystem (PS I).
5.	Students will collect and analyze data to determine the effects of various chemicals on the photosynthetic process.
6.	Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. 

Outcomes

Students who have successfully completed this exercise will:
1.	understand the process of Photosynthesis at the level of chloroplasts and photosystems within isolated plant organelles.
2.	be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time.
3.	understand how uncouplers affect oxygen production rates in terms of phosphorylation, electron transport and chemiosmosis.
4.	understand the relationship between light intensity and the rate of oxygen production in the whole electron transport process and in a single photosystem. 
5.	be able to compare coupled and uncoupled reactions between different experiments.
6.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
7.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Included with:
Lab Equipment Required: IX-TA, ISE-730 , RPC-100
Description: Photoreceptors from thylakoids – looking at carbon dioxide fixation under a variety of experimental conditions.

Goals

1.	Students will examine the process of carbon dioxide fixation using intact algal cells.
2.	Students will use a dissolved oxygen electrode and photosynthesis chamber to observe changes in the amount of dissolved oxygen in a thylakoid solution in order to examine rate of reaction. 
3.	Students will use three compounds to compare the effect on the rate of carbon dioxide fixation in intact cells:
◦	Iodoacetamide (IAA), which inhibits certain enzymes of the Calvin cycle, but should have no effect on photosynthetic electron transport. 
◦	3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), which blocks electron transport between the Qa and Qb quinones in the chain. 
◦	Methylamine (MA), which should increase the rate of electron transport by uncoupling ATP synthesis from electron transport. 
4.	Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window.

Outcomes

Students who have successfully completed this exercise will:
1.	understand the process of Photosynthesis and CO2 fixation in intact algal cells.
2.	be able to successfully use a dissolved oxygen probe to measure oxygen concentration changes over time.
3.	understand how uncouplers affect oxygen production rates in terms of phosphorylation, electron transport and chemiosmosis.
4.	understand the relationship between CO2 fixation in intact algal cells in both the dark and light; and the rate of oxygen production during the Calvin Cycle. 
5.	be able to compare oxygen production in algal cells and isolated thylakoids using the different chemicals.
6.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
7.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Included with:
Lab Equipment Required: IX-TA, IWIRE-PEAK , AC-520
Description: Uses an entire intact plant (gerbera daisy or the like) to look at photosynthesis in both light and dark conditions.

Goals

1.	Students will assemble the equipment to be able to record accurate gas analysis measurements.
2.	Students will determine the rate of oxygen production and carbon dioxide utilization in a photosynthesizing organism.
3.	Students will accurately analyze REE values.
4.	An an option, students may record:
◦	A plant a room temperature and compare to a plant at either high or low temperature.
◦	A plant contained in one concentration of CO2 compared to a plant in a higher concentration of CO2. 
◦	A plant with the light shining on the leaves to a plant in the dark. 

Outcomes

Students who have successfully completed this exercise will:
1.	determine mean REE of a plant.
2.	determine the changes in CO2 and O2 concentrations over time. 
3.	make comparisons with the values obtained under different circumstances.
4.	Use advanced analysis features to gather mathematical data.
Included with:
Lab Equipment Required: IX-TA, ISE-730
Description: Goldfish or other small fish. Calculation of metabolism by looking at the consumption of O2 based on food consumption and temperature.

Goals

1.	Students will learn to accurately weigh small organisms.
2.	Students will learn to calibrate the dissolved oxygen sensor and measure the rate of oxygen consumption over time of organisms under different metabolic conditions. 
3.	Students will collect and analyze oxygen consumption curves to determine how oxygen consumption is related to the diet or ambient temperature of an organism. 
4.	Students will continue to be successful at using the LabScribe software to move cursors, analyze data, record data to the Journal, and add functions to the Analysis window. 

Outcomes

Students who have successfully completed this exercise will:
1.	have accurately measured the weight of small animals.
2.	have successfully calibrated the dissolved oxygen sensor and recorded the oxygen consumption over time of organisms under various conditions. 
3.	after analyzing the data collected, be able to relate oxygen consumption to diet or ambient temperature.
4.	come to a conclusion about any trends shown by this experiment.
5.	feel comfortable transferring data to the Journal and interpreting that data to answer questions about their recordings.
6.	have used the functions available in the Analysis window to determine values necessary for this exercise.
Updated on April 18, 2021

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